Denani Caio, Horbach Ingrid, Setatino Bruno, Azevedo Adriana, Lima Sheila, Schwarcz Waleska, Sousa Ivanildo
Laboratório de Análise Imunomolecular, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
Programa de Pós-Graduação em Medicina Tropical, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
Hum Vaccin Immunother. 2025 Dec;21(1):2528368. doi: 10.1080/21645515.2025.2528368. Epub 2025 Jul 9.
The plaque reduction neutralization test (PRNT) is the gold standard for quantifying neutralizing antibodies owing to its high sensitivity and specificity. Widely used in seroepidemiological studies and vaccine development, PRNT is limited by labor-intensive procedures, reliance on highly skilled staff, and susceptibility to human bias during manual plaque counting. These limitations hinder reproducibility, throughput, and cross-laboratory standardization. This review summarizes recent efforts to automate PRNT workflows to address these issues. However, automation adoption is often constrained by high costs and the need for regulatory frameworks to ensure data integrity. Advancements in tailored hardware and software to diverse laboratory contexts are essential to maintaining PRNT's role in immunological assessments, bridging seroneutralizing methodologies with modern automation, and making automated methods more accessible. This review outlines the key requirements for PRNT's continued evolution.
蚀斑减少中和试验（PRNT）因其高灵敏度和特异性，是定量中和抗体的金标准。PRNT广泛应用于血清流行病学研究和疫苗开发，但受限于劳动强度大的操作程序、对高技能人员的依赖以及人工蚀斑计数过程中易受人为偏差影响。这些限制阻碍了可重复性、通量和跨实验室标准化。本综述总结了近期为使PRNT工作流程自动化以解决这些问题所做的努力。然而，自动化的采用往往受到高成本以及需要监管框架以确保数据完整性的限制。针对不同实验室环境定制硬件和软件的进展对于维持PRNT在免疫评估中的作用、弥合血清中和方法与现代自动化之间的差距以及使自动化方法更易于使用至关重要。本综述概述了PRNT持续发展的关键要求。
