Kim Colin K, Gameiro Giovana R, Simms Ava-Gaye, Lam Byron L, Rich Collin A, Wang Jianhua, Jiang Hong
University of Miami Miller School of Medicine, Miami, FL, USA.
Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Curr Eye Res. 2025 Jul 9:1-8. doi: 10.1080/02713683.2025.2527755.
To characterize retinal mitochondrial function and its relationship with intraretinal thicknesses in healthy adults.
Retinal flavoprotein fluorescence (FPF), a marker of mitochondrial function, was measured using the OcuMet Beacon (OcuSciences, Inc., Ann Arbor, MI), and a stress index (SI) was computed using Enhanced Retinal Metabolic Analysis software 2.0 (RMA). After lens compensation, mean FPF in the macula and optic nerve head (ONH) was obtained. The macular SI summarizes FPF heterogeneity, while the ONH SI reflects the extent to which ONH FPF exceeds a normative threshold. Intraretinal layer thicknesses and total retinal thickness (TRT) were measured spectral-domain OCT (AngioVue, Optovue, Inc., ver. 2018.1.0.43) using 6 x 6 mm macular scans. A total of 75 healthy adults (mean age ± SD: 56.1 ± 21.7 years; range: 23-89) were imaged.
Macular and ONH FPF were inversely correlated with TRT ( < 0.01), retinal nerve fiber layer (RNFL) thickness ( < 0.05), and ganglion cell-inner plexiform layer (GCIPL) thickness ( < 0.01). ONH FPF also showed a negative correlation with the thickness of the OPL-EZ region-defined as the area between the posterior boundary of the outer plexiform layer (OPL) and the anterior boundary of the ellipsoid zone (EZ) (r = -0.24, = 0.044)-and the photoreceptor layer (PR) (r = -0.34, = 0.003). Age was significantly associated with FPF and with several intraretinal layer thicknesses, including TRT, RNFL, GCIPL, OPL-EZ region, and PR (all < 0.05). However, after adjusting for age, associations between FPF and intraretinal thicknesses were no longer significant (all > 0.05).
This study is the first to examine the relationship between retinal mitochondrial function and intraretinal layer thicknesses in healthy adults. Findings suggest that age mediates the observed associations.
描述健康成年人视网膜线粒体功能及其与视网膜内各层厚度的关系。
使用OcuMet Beacon（OcuSciences公司，密歇根州安阿伯）测量视网膜黄素蛋白荧光（FPF），这是线粒体功能的一个指标，并使用增强视网膜代谢分析软件2.0（RMA）计算应激指数（SI）。在进行晶状体补偿后，获得黄斑和视神经乳头（ONH）的平均FPF。黄斑SI总结了FPF的异质性，而ONH SI反映了ONH FPF超过正常阈值的程度。使用6×6 mm黄斑扫描的光谱域光学相干断层扫描（AngioVue，Optovue公司，版本2018.1.0.43）测量视网膜各层厚度和视网膜总厚度（TRT）。共对75名健康成年人（平均年龄±标准差：56.1±21.7岁；范围：23 - 89岁）进行了成像。
黄斑和ONH FPF与TRT（<0.01）、视网膜神经纤维层（RNFL）厚度（<0.05）以及神经节细胞 - 内丛状层（GCIPL）厚度（<0.01）呈负相关。ONH FPF还与外丛状层（OPL）后边界与椭圆体带（EZ）前边界之间区域（定义为OPL - EZ区域）的厚度呈负相关（r = -0.24，P = 0.044），与光感受器层（PR）厚度呈负相关（r = -0.34，P = 0.003）。年龄与FPF以及包括TRT、RNFL、GCIPL、OPL - EZ区域和PR在内的多个视网膜内各层厚度显著相关（均P < 0.05）。然而，在调整年龄后，FPF与视网膜内各层厚度之间的关联不再显著（均P > 0.05）。
本研究首次探讨了健康成年人视网膜线粒体功能与视网膜内各层厚度之间的关系。研究结果表明年龄介导了所观察到的关联。
