Hesp W L, Hendriks T, Schillings P H, Lubbers E J, de Boer H H
Br J Exp Pathol. 1985 Oct;66(5):511-8.
Various histological parameters of wound repair have been studied in intestinal anastomoses in the rabbit in order to compare the healing processes in ileum and colon. The sequentional appearance of granulocytes, macrophages and fibroblasts has been studied from 3 h to 7 days after operation. Also, degree of necrosis, formation of capillaries and mucosal repair were analysed semi-quantitatively. Significant differences were observed between ileal and colonic anastomoses with respect to occurrence of granulocytes, necrosis and mucosal repair, particularly 7 days after operation. At that time, granulocytes and necrosis were virtually absent from ileal anastomoses, while mucosal integrity was restored in the majority of cases examined. In contrast, granulocytes and necrosis were still abundantly present in colonic anastomoses and mucosal repair was poor. These results support biochemical evidence that wounds in the ileum heal more rapidly than wounds in the colon. Possibly, the sustained presence of granulocytes, which are a potential source of collagenase activity, is important in this respect. Investigation of the same features of intestinal wound healing in rabbits with peritonitis induced by infection showed similar results and no differences were found between infected and non-infected animals.
为了比较兔回肠和结肠的愈合过程，已对兔肠吻合术中伤口修复的各种组织学参数进行了研究。从术后3小时至7天，对粒细胞、巨噬细胞和成纤维细胞的相继出现情况进行了研究。此外，还对坏死程度、毛细血管形成和黏膜修复进行了半定量分析。在粒细胞的出现、坏死和黏膜修复方面，回肠和结肠吻合术之间观察到显著差异，尤其是在术后7天。此时，回肠吻合处几乎没有粒细胞和坏死，而在大多数检查病例中黏膜完整性得以恢复。相比之下，结肠吻合处仍大量存在粒细胞和坏死，黏膜修复较差。这些结果支持了生化证据，即回肠伤口比结肠伤口愈合得更快。可能在这方面，作为胶原酶活性潜在来源的粒细胞持续存在很重要。对感染诱发腹膜炎的兔肠道伤口愈合相同特征的研究显示了类似结果，且在感染和未感染动物之间未发现差异。
