高胆固醇血症对大鼠实验性结肠吻合口伤口愈合的影响。
Effect of hypercholesterolemia on experimental colonic anastomotic wound healing in rats.
作者信息
Sen Meral, Anadol A Ziya, Oğuz Mehmet
机构信息
Department of Surgery, School of Medicine, Fatih University, 06500 Ankara, Turkey.
出版信息
World J Gastroenterol. 2006 Feb 28;12(8):1225-8. doi: 10.3748/wjg.v12.i8.1225.
AIM
To evaluate the mechanical and biochemical parameters of colonic anastomotic healing in hypercholesterolemic rats.
METHODS
Sixty rats were divided into two groups of 30 each according to their dietary regimens. The test group was fed with a high cholesterol-containing diet for two months while the control group had standard diet. These two groups were further divided into three subgroups consisting of ten rats each. After hypercholesterolemia was established, left colon resection and anastomosis were performed in both groups and samples from liver and abdominal aorta were taken to evaluate the systemic effects of hypercholesterolemia. Anastomotic wound healing, blow-out pressures and tissue hydroxyproline levels were evaluated.
RESULTS
The test group had a significant weight gain in two months. Microscopic examination of the abdominal aorta revealed no atherosclerotic change in none of the groups, but liver tissue specimens showed significant steatosis in the test group. Tissue hydroxyproline levels and anastomotic blow-out pressures were significantly lower in the test group than in the controls.
CONCLUSION
Hypercholesterolemia not only increases hydroxyproline levels and blow-out pressures but also worsens anastomotic wound healing.
目的
评估高胆固醇血症大鼠结肠吻合口愈合的力学和生化参数。
方法
60只大鼠根据饮食方案分为两组，每组30只。试验组喂食含高胆固醇饮食两个月，而对照组给予标准饮食。这两组再进一步分为三个亚组，每组10只大鼠。高胆固醇血症形成后，两组均进行左半结肠切除和吻合，并采集肝脏和腹主动脉样本以评估高胆固醇血症的全身影响。评估吻合口伤口愈合、破裂压力和组织羟脯氨酸水平。
结果
试验组在两个月内体重显著增加。腹主动脉的显微镜检查显示两组均无动脉粥样硬化改变，但试验组肝脏组织标本显示明显的脂肪变性。试验组的组织羟脯氨酸水平和吻合口破裂压力显著低于对照组。
结论
高胆固醇血症不仅会增加羟脯氨酸水平和破裂压力，还会使吻合口伤口愈合恶化。
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