Herawati Elisa, Titisari Rizki S, Kristin Helen, Prasasti Filosofia F T A, Agnesia Pipin, Astirin Okid P, Listyawati Shanti, Susanti Evi, Herdyastuti Nuniek, Laily Noer, Illaningtyas Fatim
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Surakarta, Indonesia.
Department of Biology, Faculty of Science and Mathematics, Universitas Diponegoro, Semarang, Indonesia.
J Complement Integr Med. 2025 Jul 10. doi: 10.1515/jcim-2025-0093.
Hydrolyzed collagen (HC) extracted from fish skin is a safe and effective native collagen alternative that can potentially protect skin from experiencing photoaging. This study is among the first to explore HC from mackerel scad () application via and approaches.
The protective effects of HC were investigated on Mouse Embryonic Fibroblasts (MEFs) induced by acute and chronic UVB, followed by testing of HC gel on UVB-induced photoaging models of mice. Cell viability test using the MTT method was performed on UVB-induced MEFs treated with HC concentrations of 10, 20, 50, and 100 g/mL. A gel form HC was applied topically in UVB-induced skin tissues of animal models and analyzed with ELISA to evaluate the inhibitory effect of MMP-1 and IL-6 expression.
analysis showed that HC (10, 20, 50 μg/mL) could increase the viability of acute UVB-induced MEFs at a 60 mJ/cm dose. In chronic UVB exposure, all HC concentrations could protect the viability of induced MEFs at 40 mJ/cm dose. HC gel effectively inhibited the increased expression of MMP-1 and IL-6 in UVB-exposed skin, which may be related to its ability to suppress the activation of MAPK and NF-κB signaling pathways. Furthermore, HC gel intervened in the development of epidermal thickening and dermal layer damage in photoaged skin.
These findings indicated that HC from mackerel scad skin effectively provided a protective effect against the photoaging process.
从鱼皮中提取的水解胶原蛋白（HC）是一种安全有效的天然胶原蛋白替代品，有可能保护皮肤免受光老化。本研究是最早通过[具体方法1]和[具体方法2]探索鲐鲅鱼中HC应用的研究之一。
研究了HC对急性和慢性紫外线B（UVB）诱导的小鼠胚胎成纤维细胞（MEF）的保护作用，随后在UVB诱导的小鼠光老化模型上测试了HC凝胶。对用浓度为10、20、50和100μg/mL的HC处理的UVB诱导的MEF进行MTT法细胞活力测试。将凝胶形式的HC局部应用于动物模型的UVB诱导的皮肤组织，并通过酶联免疫吸附测定（ELISA）分析以评估基质金属蛋白酶-1（MMP-1）和白细胞介素-6（IL-6）表达的抑制作用。
[具体分析方法]分析表明，HC（10、20、50μg/mL）可在60 mJ/cm²剂量下提高急性UVB诱导的MEF的活力。在慢性UVB照射下，所有HC浓度均可在40 mJ/cm²剂量下保护诱导的MEF的活力。HC凝胶有效抑制了UVB照射皮肤中MMP-1和IL-6表达的增加，这可能与其抑制丝裂原活化蛋白激酶（MAPK）和核因子κB（NF-κB）信号通路激活的能力有关。此外，HC凝胶干预了光老化皮肤中表皮增厚和真皮层损伤的发展。
这些发现表明，鲐鲅鱼皮中的HC有效地提供了对光老化过程的保护作用。
