衰老人类红细胞中分离纯化的血影蛋白与血红蛋白相关性的证明。

Demonstration of haemoglobin associated with isolated, purified spectrin from senescent human red cells.

作者信息

Snyder L M, Garver F, Liu S C, Leb L, Trainor J, Fortier N L

出版信息

Br J Haematol. 1985 Nov;61(3):415-9. doi: 10.1111/j.1365-2141.1985.tb02845.x.

DOI:10.1111/j.1365-2141.1985.tb02845.x

Abstract

Employing a direct and sensitive radioimmunoassay (RIA) we have confirmed the presence of haemoglobin associated with isolated, purified spectrin from senescent red cells. Haemoglobin associated with spectrin occurs in the highest amount in cells with an MCHC greater than 36 g/dl and is approximately 3% of the total spectrin extract. Spectrin from the young cells had the least haemoglobin, while an intermediate amount was found in unfractionated, whole red cells. The RIA results were in close approximation with estimation of the haemoglobin-spectrin complex obtained by carefully integrating the Coomassie blue stain profiles from 4% SDS PAGE in densitometric scans from isolated spectrin.

摘要

我们采用一种直接且灵敏的放射免疫分析法（RIA），证实了衰老红细胞中分离纯化的血影蛋白存在与之相关的血红蛋白。与血影蛋白相关的血红蛋白在平均红细胞血红蛋白浓度（MCHC）大于36 g/dl的细胞中含量最高，约占血影蛋白提取物总量的3%。年轻细胞中的血影蛋白所含血红蛋白最少，而在未分级的全红细胞中则发现有中等含量。放射免疫分析结果与通过对分离血影蛋白的4%十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS PAGE）考马斯亮蓝染色图谱进行光密度扫描并仔细积分得到的血红蛋白-血影蛋白复合物估计值非常接近。