Pelech Petr, Navarro Paula P, Vettiger Andrea, Chao Luke H, Allolio Christoph
Mathematical Institute, Faculty of Mathematics and Physics, Charles University, Praha 18675, Czech Republic.
Department of Fundamental Microbiology, University of Lausanne, Quartier UNIL-Sorge, Lausanne 1015, Switzerland.
Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2424441122. doi: 10.1073/pnas.2424441122. Epub 2025 Jul 9.
In order to proliferate, bacteria must remodel their cell wall at the division site. The division process is driven by the enzymatic activity of peptidoglycan synthases and hydrolases around the constricting Z-ring. We introduce a morphoelastic model that correctly reproduces the shape of the division site during the constriction and septation phases of . In the model, mechanical stress directs the transformation of the bacterial wall. The two constants associated with growth and remodeling respectively are its only adjustable parameters. Different morphologies, corresponding either to mutant or wild type cells, are recovered as a function of the remodeling parameter. In addition, a plausible range for the cell stiffness and turgor pressure was determined by comparing numerical simulations with bacterial cell plasmolysis data.
为了增殖，细菌必须在分裂位点重塑其细胞壁。分裂过程由收缩的Z环周围的肽聚糖合成酶和水解酶的酶活性驱动。我们引入了一个形态弹性模型，该模型能够正确再现细菌在收缩和隔膜形成阶段分裂位点的形状。在该模型中，机械应力引导细菌壁的转变。分别与生长和重塑相关的两个常数是其仅有的可调参数。作为重塑参数的函数，可以恢复对应于突变体或野生型细胞的不同形态。此外，通过将数值模拟与细菌细胞质壁分离数据进行比较，确定了细胞刚度和膨压的合理范围。
