Chen Xuan, Zhang Yu, Wang Xiaohong, Wu Zhenling, Ji Qiurong, He Tingli, Zhu Kaina, Zhang Feng Shuo, Hou Shengzhen, Gui Linsheng
College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai, China.
Guinan Animal Disease Prevention and Control Center, Guinan County, Qinghai, China.
Genomics. 2025 Jul 7;117(5):111078. doi: 10.1016/j.ygeno.2025.111078.
Previous research studies confirmed that resveratrol (RES) and β-hydroxy-β-methyl butyric acid (HMB) regulated the glucolipid metabolism. However, the mechanism underlying of RES and HMB on subcutaneous fat remains unclear in ruminant. This study investigated the effects of dietary RES and HMB supplementation on immune, oxidative, and morphological changes in subcutaneous fat of Tibetan sheep using transcriptomics and metabolomics analysis. One hundred and twenty male Tibetan lambs of similar initial weight (15.5 ± 0.14 kg) were randomly divided into four groups of 30 lambs each: 1) H group (basal diet without RES or HMB); 2) H-RES group (1.5 g/day RES); 3) H-HMB group (1250 mg/day HMB); and 4) H-RES-HMB group (1.5 g/day RES and 1250 mg/day HMB). The study included a pre-test period of 10 days and a formal experimental period of 90 days. The results showed that, the supplementation of RES and HMB in combination reduced the backfat thickness and adipocyte diameter (P < 0.05). Notably, dietary RES and HMB significantly influenced the composition of fatty acid in subcutaneous fat, including increased monounsaturated fatty acid (MUFA), such as C16:1 N7, C18:1 N9, C20:1 N9, C22:1 N9, C24:1 N9, C24:1 N, and decreased saturated fatty acid (SFA), such as C8:0, C10:0, C11:0, C24:0. The H-RES-HMB group enriched in the lipid-related signaling pathways, including "linoleic acid", "arachidonic acid", "tyrosine" and "retinol". The transcriptome and metabolome indicated ten key genes including PLA2G5, PLA2G2D, PTGES, TBXAS1, ALOX5, MAOA, TYR, UGT1A4, RDH5, and DHRS9) and five metabolites including PC(33:4), LPC(22:0), LPC(15:0), LPC(16:1), PC(42:8) were identified as key biomarkers. In conclusion, dietary RES and HMB supplementation regulated the lipid metabolism by modulating gene transcription and metabolism in Tibetan sheep.
先前的研究证实，白藜芦醇（RES）和β-羟基-β-甲基丁酸（HMB）可调节糖脂代谢。然而，在反刍动物中，RES和HMB对皮下脂肪作用的潜在机制仍不清楚。本研究采用转录组学和代谢组学分析方法，研究日粮添加RES和HMB对藏羊皮下脂肪免疫、氧化和形态变化的影响。120只初始体重相近（15.5±0.14千克）的雄性藏羊羔羊被随机分为四组，每组30只：1）H组（不添加RES或HMB的基础日粮）；2）H-RES组（1.5克/天RES）；3）H-HMB组（1250毫克/天HMB）；4）H-RES-HMB组（1.5克/天RES和1250毫克/天HMB）。研究包括10天的预试验期和90天的正式试验期。结果表明，RES和HMB联合添加可降低背膘厚度和脂肪细胞直径（P<0.05）。值得注意的是，日粮RES和HMB显著影响皮下脂肪中脂肪酸的组成，包括单不饱和脂肪酸（MUFA）增加，如C16:1 N7、C18:1 N9、C20:1 N9、C22:1 N9、C24:1 N9、C24:1 N，饱和脂肪酸（SFA）减少，如C8:0、C10:0、C11:0、C24:0。H-RES-HMB组富含脂质相关信号通路，包括“亚油酸”、“花生四烯酸”、“酪氨酸”和“视黄醇”。转录组和代谢组表明，包括PLA2G5、PLA2G2D、PTGES、TBXAS1、ALOX5、MAOA、TYR、UGT1A4、RDH5和DHRS9在内的10个关键基因以及包括PC(33:4)、LPC(22:0)、LPC(15:0)、LPC(16:1)、PC(42:8)在内的5种代谢物被鉴定为关键生物标志物。总之，日粮添加RES和HMB通过调节藏羊的基因转录和代谢来调节脂质代谢。
