Wei Jian, Hu Kunshen, Liu Menglong, Liu Yali, Tian Wang, Zhou Yue, Fan Liu-Min, Wang Yizhou, Wang Yin
Institute of Ecology, College of Urban and Environmental Sciences, State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Peking University, Beijing, China.
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Nat Plants. 2025 Jul 9. doi: 10.1038/s41477-025-02026-5.
In the leaves of most herbaceous plants, stomata exist in both the adaxial (upper) and abaxial (lower) surfaces. Many previous studies have reported that stomata on the abaxial surface are more responsive to light than those on the adaxial surface. However, the underlying molecular mechanism is still unclear. Here, by examining the model plants Arabidopsis and tobacco, we confirmed that the distinct feature occurred at the guard cell level. Next, with both single-cell RNA sequencing and transcriptome sequencing, we compared gene expression patterns of adaxial and abaxial guard cells and highlighted the possibility of different utilization of potassium ion (K) channels. Via in silico OnGuard simulation and genetic modifications, we found that adaxial and abaxial guard cells rely on different K channels, which control K influx for stomatal opening. The present study provides insights into understanding the distinct stomatal light response of different leaf surfaces.
在大多数草本植物的叶片中，气孔存在于近轴（上）表面和远轴（下）表面。许多先前的研究报道，远轴表面的气孔比近轴表面的气孔对光更敏感。然而，其潜在的分子机制仍不清楚。在这里，通过研究模式植物拟南芥和烟草，我们证实了这种差异特征发生在保卫细胞水平。接下来，通过单细胞RNA测序和转录组测序，我们比较了近轴和远轴保卫细胞的基因表达模式，并强调了钾离子（K）通道不同利用方式的可能性。通过计算机模拟OnGuard和基因改造，我们发现近轴和远轴保卫细胞依赖不同的钾通道，这些通道控制钾离子内流以实现气孔开放。本研究为理解不同叶表面独特的气孔光反应提供了见解。
