The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao, 266237, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Nat Commun. 2024 Jun 14;15(1):5081. doi: 10.1038/s41467-024-49377-9.
Stomatal movement is vital for plants to exchange gases and adaption to terrestrial habitats, which is regulated by environmental and phytohormonal signals. Here, we demonstrate that hydrogen peroxide (HO) is required for light-induced stomatal opening. HO accumulates specifically in guard cells even when plants are under unstressed conditions. Reducing HO content through chemical treatments or genetic manipulations results in impaired stomatal opening in response to light. This phenomenon is observed across different plant species, including lycopodium, fern, and monocotyledonous wheat. Additionally, we show that HO induces the nuclear localization of KIN10 protein, the catalytic subunit of plant energy sensor SnRK1. The nuclear-localized KIN10 interacts with and phosphorylates the bZIP transcription factor bZIP30, leading to the formation of a heterodimer between bZIP30 and BRASSINAZOLE-RESISTANT1 (BZR1), the master regulator of brassinosteroid signaling. This heterodimer complex activates the expression of amylase, which enables guard cell starch degradation and promotes stomatal opening. Overall, these findings suggest that HO plays a critical role in light-induced stomatal opening across different plant species.
气孔运动对于植物进行气体交换和适应陆地生境至关重要，其受到环境和植物激素信号的调控。在这里，我们证明了过氧化氢（HO）是光诱导气孔开放所必需的。即使在植物处于非胁迫条件下，HO 也会特异性地在保卫细胞中积累。通过化学处理或遗传操作降低 HO 含量会导致植物对光响应的气孔开放受损。这种现象在不同的植物物种中都有观察到，包括石松、蕨类植物和单子叶植物小麦。此外，我们还表明，HO 诱导 KIN10 蛋白（植物能量传感器 SnRK1 的催化亚基）的核定位。核定位的 KIN10 与 bZIP 转录因子 bZIP30 相互作用并使其磷酸化，导致 bZIP30 和 BRASSINAZOLE-RESISTANT1（BZR1）（油菜素内酯信号的主要调节因子）形成异二聚体。这种异二聚体复合物激活了淀粉酶的表达，从而使保卫细胞淀粉降解并促进气孔开放。总的来说，这些发现表明 HO 在不同植物物种的光诱导气孔开放中起着关键作用。
