用于在培养的果蝇细胞中滴定蟋蟀麻痹病毒的终点稀释法和噬斑测定法。

End-point dilution and plaque assay methods for titration of cricket paralysis virus in cultured Drosophila cells.

作者信息

Scotti P D

出版信息

J Gen Virol. 1977 May;35(2):393-6. doi: 10.1099/0022-1317-35-2-393.

Abstract

Cricket paralysis virus, an insect picorna-like virus, caused a distinct c.p.e. in cultured Drosophila melanogaster cells, allowing the development of titration methods based on end-point dilution or plaque assay methods. The end-point dilution (TCD50) method is more sensitive and economical than plaque assays and is easily scored. The data indicate a minimum infectivity/particle ratio of about 1/2000.

摘要

蟋蟀麻痹病毒，一种昆虫类微小核糖核酸病毒，在培养的黑腹果蝇细胞中引起明显的细胞病变效应，从而使得基于终点稀释或空斑测定法的滴定方法得以发展。终点稀释（TCD50）法比空斑测定法更灵敏且经济，并且易于评分。数据表明最小感染性/粒子比率约为1/2000。