• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于在培养的果蝇细胞中滴定蟋蟀麻痹病毒的终点稀释法和噬斑测定法。

End-point dilution and plaque assay methods for titration of cricket paralysis virus in cultured Drosophila cells.

作者信息

Scotti P D

出版信息

J Gen Virol. 1977 May;35(2):393-6. doi: 10.1099/0022-1317-35-2-393.

DOI:10.1099/0022-1317-35-2-393
PMID:406358
Abstract

Cricket paralysis virus, an insect picorna-like virus, caused a distinct c.p.e. in cultured Drosophila melanogaster cells, allowing the development of titration methods based on end-point dilution or plaque assay methods. The end-point dilution (TCD50) method is more sensitive and economical than plaque assays and is easily scored. The data indicate a minimum infectivity/particle ratio of about 1/2000.

摘要

蟋蟀麻痹病毒，一种昆虫类微小核糖核酸病毒，在培养的黑腹果蝇细胞中引起明显的细胞病变效应，从而使得基于终点稀释或空斑测定法的滴定方法得以发展。终点稀释（TCD50）法比空斑测定法更灵敏且经济，并且易于评分。数据表明最小感染性/粒子比率约为1/2000。

相似文献

1
End-point dilution and plaque assay methods for titration of cricket paralysis virus in cultured Drosophila cells.用于在培养的果蝇细胞中滴定蟋蟀麻痹病毒的终点稀释法和噬斑测定法。
J Gen Virol. 1977 May;35(2):393-6. doi: 10.1099/0022-1317-35-2-393.
2
The replication and titration of iridescent virus type 22 in Spodoptera frugiperda cells.
J Gen Virol. 1978 Jan;38(1):175-8. doi: 10.1099/0022-1317-38-1-175.
3
Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus.核型多角体病毒在草地贪夜蛾连续细胞培养物中的复制：病毒的纯化、感染性测定及生长特性
J Virol. 1974 Oct;14(4):934-44. doi: 10.1128/JVI.14.4.934-944.1974.
4
Serological relations between twelve small RNA viruses of insects.十二种昆虫小RNA病毒之间的血清学关系。
J Gen Virol. 1976 Apr;31(1):131-4. doi: 10.1099/0022-1317-31-1-131.
5
The production of cricket paralysis virus in suspension cultures of insect cell lines.蟋蟀麻痹病毒在昆虫细胞系悬浮培养物中的产生。
J Invertebr Pathol. 1996 Sep;68(2):109-12. doi: 10.1006/jipa.1996.0067.
6
Plaque assay for black beetle virus.黑甲虫病毒的蚀斑测定法。
J Virol. 1984 Jul;51(1):251-3. doi: 10.1128/JVI.51.1.251-253.1984.
7
In vitro cultivation of testicular cysts of the greater wax moth and multiplication of the associated nuclear polyhedrosis virus.大蜡螟睾丸囊肿的体外培养及相关核型多角体病毒的增殖
J Invertebr Pathol. 1975 Jul;26(1):7-10. doi: 10.1016/0022-2011(75)90162-7.
8
Infectivity assays for the Autographa californica nuclear polyhedrosis virus using Spodoptera littoralis cells.
J Virol Methods. 1985 Jan;10(1):1-10. doi: 10.1016/0166-0934(85)90082-5.
9
Some biophysical properties of virus present in tissue cultures infected with the nuclear polyhedrosis virus of Trichoplusia ni.
J Gen Virol. 1974 Jan;22(1):143-6. doi: 10.1099/0022-1317-22-1-143.
10
[Host range of drosophila melanogaster C virus among diptera and lepidoptera (author's transl)].黑腹果蝇C病毒在双翅目和鳞翅目昆虫中的宿主范围（作者译）
Ann Microbiol (Paris). 1976 May-Jun;127(4):529-44.

引用本文的文献

1
The signal peptide of BmNPV GP64 activates the ERAD pathway to regulate heterogeneous secretory protein expression.家蚕核型多角体病毒 GP64 的信号肽通过激活内质网相关降解途径调控异源分泌蛋白表达。
Microb Cell Fact. 2024 Oct 18;23(1):284. doi: 10.1186/s12934-024-02534-7.
2
The circular RNA circATP8B(2) regulates ROS production and antiviral immunity in Drosophila.环状 RNA circATP8B(2)调控果蝇中 ROS 产生和抗病毒免疫。
Cell Rep. 2024 Apr 23;43(4):113973. doi: 10.1016/j.celrep.2024.113973. Epub 2024 Mar 19.
3
Digital plasmonic nanobubble detection for rapid and ultrasensitive virus diagnostics.
数字等离子体纳米气泡检测用于快速和超灵敏的病毒诊断。
Nat Commun. 2022 Mar 30;13(1):1687. doi: 10.1038/s41467-022-29025-w.
4
Enterovirus 71 Neutralizing Antibodies Seroepidemiological Research among Children in Guangzhou, China between 2014 and 2015: A Cross-Sectional Study.2014年至2015年中国广州儿童肠道病毒71型中和抗体血清流行病学研究：一项横断面研究
Int J Environ Res Public Health. 2017 Mar 20;14(3):319. doi: 10.3390/ijerph14030319.
5
The Toll-dorsal pathway is required for resistance to viral oral infection in Drosophila.在果蝇中，Toll-背侧通路是抵抗病毒口腔感染所必需的。
PLoS Pathog. 2014 Dec 4;10(12):e1004507. doi: 10.1371/journal.ppat.1004507. eCollection 2014 Dec.
6
Novel genetic reassortants in H9N2 influenza A viruses and their diverse pathogenicity to mice.新型 H9N2 流感 A 病毒的基因重配及其对小鼠的不同致病性。
Virol J. 2011 Nov 4;8:505. doi: 10.1186/1743-422X-8-505.
7
Rapid titration of measles and other viruses: optimization with determination of replication cycle length.快速滴定麻疹和其他病毒：通过确定复制周期长度进行优化。
PLoS One. 2011;6(9):e24135. doi: 10.1371/journal.pone.0024135. Epub 2011 Sep 7.
8
Construction and characterization of insect cell-derived influenza VLP: cell binding, fusion, and EGFP incorporation.昆虫细胞衍生的流感病毒样颗粒的构建与表征：细胞结合、融合及绿色荧光蛋白掺入
J Biomed Biotechnol. 2010;2010:506363. doi: 10.1155/2010/506363. Epub 2010 Dec 9.
9
The Imd pathway is involved in antiviral immune responses in Drosophila.Imd 途径参与果蝇的抗病毒免疫反应。
PLoS One. 2009 Oct 15;4(10):e7436. doi: 10.1371/journal.pone.0007436.
10
Characterization of cricket paralysis virus-induced polypeptides in Drosophila cells.果蝇细胞中蟋蟀麻痹病毒诱导的多肽的特性分析。
J Virol. 1980 Jan;33(1):1-9. doi: 10.1128/JVI.33.1.1-9.1980.