Zhang Dingmei, Chen Yan, Chen Xiashi, He Zhenjian, Zhu Xun, Hao Yuantao
School of Public Health, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China.
Medical College of Shaoguan University, No.1 Xinhuanan Road, Shaoguan 512000, China.
Int J Environ Res Public Health. 2017 Mar 20;14(3):319. doi: 10.3390/ijerph14030319.
A hand-foot-mouth disease outbreak occurred in 2014 around Guangdong. The purpose of this study was investigating the status and susceptibility of infectious neutralizing antibodies to enterovirus 71 among children so as to provide scientific evidence for the population immunity level of hand-foot-mouth disease and prepare for enterovirus 71 vaccination implementation. Serum specimens were collected from children in communities from January 2014 to March 2015 in Guangzhou. A total of 197 serum samples from children 1-5 years old were collected for this cross-sectional study via non-probabilistic sampling from the database of Chinese National Science and Technique Major Project. Neutralization activity was measured via micro neutralization test in vitro. The positive rate of enterovirus 71 neutralizing antibodies was 59.4%, whereas the geometric mean titre was 1:12.7. A statistically significant difference in true positive rates was found between different age groups but not between different genders. Being the most susceptible population of hand-foot-mouth disease, children under 3 years of age are more likely to be infected with enterovirus 71, and the immunity of children increases with increasing age. Further cohort studies should be conducted, and measures for prevention and vaccination should be taken.
2014年广东周边地区发生了手足口病疫情。本研究旨在调查儿童肠道病毒71型感染中和抗体的状况及易感性，为手足口病的人群免疫水平提供科学依据，并为肠道病毒71型疫苗接种工作的实施做好准备。于2014年1月至2015年3月期间收集广州社区儿童的血清标本。通过非概率抽样从中国国家科技重大专项数据库中收集了197份1-5岁儿童的血清样本用于本横断面研究。采用体外微量中和试验检测中和活性。肠道病毒71型中和抗体阳性率为59.4%，几何平均滴度为1:12.7。不同年龄组之间的真阳性率存在统计学显著差异，而不同性别之间则无差异。3岁以下儿童作为手足口病最易感人群，更易感染肠道病毒71型，且儿童免疫力随年龄增长而增强。应进一步开展队列研究，并采取预防和接种措施。
