补充CNCM I-1079对健康日本黑犊牛粪便微生物群的影响：一项田间试验。

Effects of supplementation with CNCM I-1079 on the fecal microbiota of healthy young Japanese black calves: A field trial.

作者信息

Mori Kazusa, Uchiumi Asato, Yamamoto Kai, Shimizu Yuki, Ueda Risa, Fukazawa Hiroyuki, Ohtsuka Hiromichi

机构信息

School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyo-dai-midorimachi, Ebetsu, Hokkaido 069-8501, Japan (Mori, Ohtsuka); Lallemand-Biotech Co., Ltd., JRE Shiba 2-chome Daimon Bldg., 3-3 Shiba 2-chome, Minato-ku, Tokyo 105-0014, Japan (Mori, Fukazawa); Nosan Corporation, 46F Yokohama Landmark Tower, 2-2-1, Minatomirai, Nishi-ku, Yokohama, Kanagawa 220-8146, Japan (Uchiumi, Yamamoto, Shimizu, Ueda); Nosan Farm Co., Ltd. 7-32 Osarushi, Otofukecho, Katogun, Hokkaido 080-0271, Japan (Yamamoto); Schools of Agriculture and Animal Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11, Inadachonishi, Obihiro, Hokkaido, Japan (Ohtsuka).

出版信息

Can J Vet Res. 2025 Jul 1;89(3):91-99. eCollection 2025 Jul.

PMID:40636000

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12236094/

Abstract

The effects of more than 1 month of supplementation with on the gut microbiota of healthy young calves are unclear. The objective of this study was to describe the effects of 7 wk of supplementation with CNCM I-1079 on the fecal bacterial microbiota of healthy young Japanese black calves. Calves in the group (SB group) were fed 2.0 × 10 CFUs/day of CNCM I-1079 in milk replacer starting at 2 wk of age. Fecal samples were collected and the V3 and V4 regions of 16S rRNA were sequenced. The SB group exhibited significantly higher fecal alpha diversity at 9 wk of age (7 wk after starting supplementation) than the untreated control group. Beta diversity differed at 6 and 9 wk of age (4 and 7 wk after supplementation). Analysis of the composition of microbiomes revealed that calves in the SB group had higher fecal levels of _RF39 than the control group at 9 wk of age. Linear discriminant analysis of effect sizes revealed higher levels of at 3 wk of age (1 wk after) and of _RF39 and at 9 wk of age (7 wk after) in the SB group than in the control group. Furthermore, a higher abundance of bacteria-producing short-chain fatty acids (SCFAs) was observed at 9 wk of age than at 6 wk in the SB group. These findings indicate that CNCM I-1079 can increase bacterial alpha diversity and might increase the abundance of SCFA-producing bacteria in the gut of healthy young calves.

摘要

超过1个月补充[具体物质未给出]对健康幼龄犊牛肠道微生物群的影响尚不清楚。本研究的目的是描述7周补充CNCM I-1079对健康幼龄日本黑犊牛粪便细菌微生物群的影响。[具体物质未给出]组（SB组）的犊牛从2周龄开始在代乳粉中每天饲喂2.0×10 CFUs的CNCM I-1079。收集粪便样本并对16S rRNA的V3和V4区域进行测序。SB组在9周龄（开始补充后7周）时粪便α多样性显著高于未处理的对照组。β多样性在6周龄和9周龄（补充后4周和7周）时有所不同。微生物群落组成分析显示，SB组犊牛在9周龄时粪便中_RF39的水平高于对照组。效应大小的线性判别分析显示，SB组在3周龄（1周后）时[具体物质未给出]的水平以及在9周龄（7周后）时_RF39和[具体物质未给出]的水平高于对照组。此外，SB组在9周龄时观察到产生短链脂肪酸（SCFAs）的细菌丰度高于6周龄时。这些发现表明，CNCM I-1079可以增加细菌α多样性，并可能增加健康幼龄犊牛肠道中产生SCFA的细菌丰度。