School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyo-dai-midorimachi, Ebetsu, Hokkaido 069-8501, Japan (Mori, Kosenda, Fukuhara, Kure, Ohtsuka); Lallemand-Biotech Co., Ltd. JRE Shiba 2-chome Daimon Bldg. 3-3 Shiba 2-chome, Minato-ku, Tokyo 105-0014, Japan (Mori, Fukazawa); Nosan Corporation, 46F Yokohama Landmark Tower, 2-2-1, Minatomirai, Nishi-ku, Yokohama, Kanagawa 220-8146, Japan (Uchiumi, Yamamoto, Shimizu, Ueda); Nosan Farm Co., Ltd. 7-32 Osarushi, Otofukecho, Katogun, Hokkaido 080-0271, Japan (Yamamoto).
Can J Vet Res. 2023 Jul;87(3):237-242.
group (SB group) calves were fed 2.0 × 10 CFU/day of in milk replacer after 2 wk of age. All calves received inactivated vaccine for , and at 3 wk of age and 3 wk later. After vaccination, the SB group calves showed significantly higher (mean difference: 1.56-fold) antibody titer against than the control group. The number of calves with the antibody titer above the cut-off value for of the SB group was significantly higher than that of the control, and the percentage was twice as high. In addition, the mRNA transcription of and in peripheral blood mononuclear cells at the booster of the SB group was significantly higher than those of the control. In conclusion, may have positively affected immune responses to the inactivated multi-bacterial vaccine in young calves in the field.
试验组（SB 组）犊牛在 2 周龄后于代乳料中摄入 2.0×10 CFU/天的 。所有犊牛在 3 周龄和 3 周后都接受了灭活的 、 和 疫苗。接种疫苗后，SB 组犊牛针对 的抗体滴度显著升高（平均差异：1.56 倍）。SB 组抗体滴度高于 的截断值的犊牛数量明显高于对照组，且百分比是对照组的两倍。此外，在加强免疫时，SB 组外周血单个核细胞中 和 的 mRNA 转录显著高于对照组。总之， 可能对现场年轻犊牛的多联灭活疫苗免疫反应产生了积极影响。
