Yamamoto Kazuhiko, Inui Takayuki, Kawano Noriaki, Tamura Takayuki, Sakurai Miki, Jinbou Tomokazu, Komatsu Katsuko, Yoshimatsu Kayo
Tsukuba Division, Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition.
Center for Medicinal Plant Resources, Toyama Prefectural Institute for Pharmaceutical Research.
Plant Biotechnol (Tokyo). 2025 Jun 25;42(2):179-183. doi: 10.5511/plantbiotechnology.25.0120a.
, the roots of which are used as a crude drug, is one of the most widely used and important medicinal plants. The long cultivation period and low proliferation rate of makes it difficult to propagate large numbers of plants within a short period. We developed a bio-nursery system using plant tissue culture techniques to contribute to the supply of seeds and seedlings in Japan. Here, we report on the improved tissue culture and acclimation conditions for a more stable and efficient bio-nursery system. We investigated the effect of culture conditions on shoot proliferation and the effect of calcium concentration during root induction and acclimation of cultured plantlets. The results demonstrated that the number of shoots increased under the 15/5°C diurnal temperature changing treatment [15°C, 12 h light (fluorescent light, 80-130 µmol m s)/5°C, 12 h dark] compared to a constant temperature of 15°C. A higher calcium concentration (6 mM Ca) during root induction resulted in more vigorous growth after transplantation to the soil. In addition, it was found that planting in a closed greenhouse at a constant temperature of 20°C after cold treatment was suitable for acclimation of cultured plantlets. These findings are expected to contribute to the future seedling supply of
其根用作药材，是使用最广泛且最重要的药用植物之一。其较长的栽培周期和较低的增殖率使得在短时间内大量繁殖植株变得困难。我们利用植物组织培养技术开发了一种生物育苗系统，以助力日本的种子和幼苗供应。在此，我们报告了用于更稳定、高效生物育苗系统的改良组织培养和驯化条件。我们研究了培养条件对芽增殖的影响以及钙浓度在培养苗生根诱导和驯化过程中的作用。结果表明，与15°C恒温相比，在15/5°C昼夜温度变化处理[15°C，12小时光照（荧光灯，80 - 130 μmol·m⁻²·s⁻¹）/5°C，12小时黑暗]下芽的数量增加。生根诱导期间较高的钙浓度（6 mM Ca）导致移植到土壤后生长更旺盛。此外，发现冷处理后在20°C恒温的封闭温室中种植适合培养苗的驯化。这些发现有望为未来的幼苗供应做出贡献
