Laboratory of Vegetable and Ornamental Horticulture, Faculty of Agriculture, Shimane University, Nishikawatsu-cho 1060, 690, Matsue, Japan.
Plant Cell Rep. 1989 Apr;8(4):243-6. doi: 10.1007/BF00778543.
A procedure for the clonal propagation ofPaeonia lactiflora Pall. cvs. Takinoyosooi and Sarah Bernhardt through shoot tip culture is described. Half strength Murashige and Shoog (1962) medium supplemented with 0.5 mg/l 6-benzylaminopurine plus 1 mg/l gibberellic acid promoted formation and growth of axillary buds. Continuous shoot multiplication was achieved by vertically splitting the shoot axis and subsequent division of elongated axillary shoots every 36 days. High frequency (57-100%) of rooting was obtained on paper-bridge liquid medium supplemented with 1 mg/l indole-3-butyric acid. Half of the rooted plantlets were established on porous soil. Thus, 700 and 300 plants of cv. Takinoyosooi and Sarah Bernhardt could be theoretically obtained from a single bud in one year.
描述了芍药品种‘塔金约索伊’和‘莎拉·伯恩哈特’通过茎尖培养进行无性繁殖的过程。在半强度 Murashige 和 Shoog（1962）培养基中添加 0.5mg/l6-苄基腺嘌呤和 1mg/l 赤霉素，促进了腋芽的形成和生长。通过将茎轴垂直分裂，并每隔 36 天对伸长的腋芽进行分割，实现了连续的芽增殖。在添加 1mg/l 吲哚丁酸的纸桥液体培养基上，生根频率高（57-100%）。将一半生根的组培苗移栽到多孔土壤中。因此，理论上从一个芽一年可以获得 700 株和 300 株‘塔金约索伊’和‘莎拉·伯恩哈特’品种的植株。
