BACKGROUND: The therapeutic potential of herbs in treating various diseases is gaining recognition due to their promising outcomes and minimal side effects. One such herb, , is renowned for its medicinal properties, particularly due to its extract, Thymoquinone (TQ), which demonstrates anti-inflammatory, anti-oxidant, antimicrobial, analgesic, and immune-modulating effects. Green synthesis methods for nano-sized particles are considered environmentally friendly and have been proven effective given their size, higher surface-to-volume ratio, and enhanced physical and mechanical properties. This study aims to investigate a novel approach in the green synthesis of silver nanoparticles of and 0.1% TQ and to assess their anti-inflammatory and antioxidant activities. MATERIALS AND METHODS: The seeds of NS were used to green synthesize silver nanoparticles. The anti-inflammatory effects of the green synthesized particles were tested using egg albumin and bovine serum assays, and their anti-oxidant actions were assessed using 2,2-Diphenyl-1-picrylhydrazyl denaturation assay and HO assay. RESULTS: The novel formulation of -mediated silver nanoparticles with 0.1% TQ exhibits 76% anti-inflammatory activity and 80%-87% anti-oxidant properties. CONCLUSION: Our results suggest that the silver nanoparticles mediated from with 0.1% TQ extract could serve as an effective herbal adjunct for treating periodontal infections because of its potent anti-inflammatory and anti-oxidant properties.