Tesseur Coralie, Remy Ophélie, Laloux Géraldine, Santin Yoann
de Duve Institute, UCLouvain, Brussels, Belgium.
ITQB NOVA, NOVA University of Lisbon, Lisbon, Portugal.
Methods Mol Biol. 2025;2953:71-80. doi: 10.1007/978-1-0716-4694-6_5.
Protein-protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein networks in bacteria. In this chapter, we outline a standardized protocol for implementing miniTurbo-dependent proximity labeling in bacterial models. This approach enables the unbiased identification of interacting and proximal proteins under native conditions, providing valuable insights into bacterial cell physiology.
蛋白质-蛋白质相互作用在许多细菌细胞过程中起着至关重要的作用。基于miniTurbo的邻近标记已成为鉴定细菌体内蛋白质网络的强大工具。在本章中，我们概述了在细菌模型中实施依赖miniTurbo的邻近标记的标准化方案。这种方法能够在天然条件下无偏见地鉴定相互作用和近端蛋白质，为细菌细胞生理学提供有价值的见解。
