Welsch C W, DeHoog J V, O'Connor D H, Sheffield L G
Cancer Res. 1985 Dec;45(12 Pt 1):6147-54.
Twenty-one-day-old female BALB/c mice were divided into three groups and fed a diet containing 0, 5, and 20% fat (corn oil). Ten days prior to sacrifice, one-half of the mice were given injections daily with saline (0.9% NaCl solution), and the remaining half, with 17 beta-estradiol (1 microgram) and progesterone (1 mg). After 3 mo on diet and 10 days of saline or estradiol:progesterone treatments, all mice were sacrificed, and mammary glands were excised and prepared for whole-mount evaluation (No. 4 glands), [3H]thymidine-autoradiographic analysis (No. 2 glands), and organ culture analysis (No. 2 glands). Whole-mount evaluation involved a rating for ductal and alveolar development on a scale of 1 to 6. [3H]Thymidine-autoradiographic analysis consisted of determining the total number of labeled epithelial cells per anterior 3 mm of gland. Organ culture analysis consisted of placing one gland of each gland pair in basal tissue culture medium, and the contralateral gland was placed in basal medium plus mammogenic hormones. These glands were cultured for 6 days and then analyzed for development by whole-mount evaluation (scale, 1 to 6) and for epithelial area (mm2) (via computer image analysis). In saline- and estradiol:progesterone-treated mice, there was a significant linear increase in the number of [3H]thymidine-labeled mammary epithelial cells as the fat content of the diet increased from 0 to 5 to 20% (P less than 0.05). In saline- and estradiol:progesterone-treated mice, mammary gland development (assessed by whole-mount evaluation) was increased as the fat content of the diet increased from 0 to 5% (P less than 0.05). In saline-treated mice, no significant difference in mammae development was observed between mice fed 5 or 20% fat diets; in estradiol:progesterone-treated mice, mammae development was marginally increased in mice fed the 20% fat diet compared to mice fed the 5% fat diet (P approximately 0.07).(ABSTRACT TRUNCATED AT 400 WORDS)
将21日龄的雌性BALB/c小鼠分为三组，分别喂食含0%、5%和20%脂肪（玉米油）的饮食。在处死前10天，一半小鼠每天注射生理盐水（0.9%氯化钠溶液），另一半注射17β-雌二醇（1微克）和孕酮（1毫克）。在饮食3个月以及生理盐水或雌二醇与孕酮处理10天后，处死所有小鼠，切除乳腺并制备用于整体评估（4号腺体）、[3H]胸腺嘧啶核苷放射自显影分析（2号腺体）和器官培养分析（2号腺体）。整体评估包括对导管和腺泡发育进行1至6级评分。[3H]胸腺嘧啶核苷放射自显影分析包括确定腺体前3毫米内标记上皮细胞的总数。器官培养分析包括将每对腺体中的一个腺体置于基础组织培养基中，对侧腺体置于基础培养基加促乳腺激素中。这些腺体培养6天，然后通过整体评估（1至6级）分析发育情况，并通过计算机图像分析测量上皮面积（平方毫米）。在注射生理盐水和注射雌二醇与孕酮的小鼠中，随着饮食中脂肪含量从0%增加到5%再增加到20%，[3H]胸腺嘧啶核苷标记的乳腺上皮细胞数量呈显著线性增加（P<0.05）。在注射生理盐水和注射雌二醇与孕酮的小鼠中，随着饮食中脂肪含量从0%增加到5%，乳腺发育（通过整体评估）增加（P<0.05）。在注射生理盐水的小鼠中，喂食5%或20%脂肪饮食的小鼠之间乳腺发育无显著差异；在注射雌二醇与孕酮的小鼠中，与喂食5%脂肪饮食的小鼠相比，喂食20%脂肪饮食的小鼠乳腺发育略有增加（P约为0.07）。（摘要截短至400字）
