Mei Yang, Cheng Ziqi, Lu Yueqi, Wu Shiyi, Chen Xi
College of Plant Protection, Jilin Agricultural University, Changchun, 130118, China.
Yangtze Delta Region Institute (Quzhou), University of Electronic Science and Technology of China, Quzhou, 324000, China.
Sci Data. 2025 Jul 10;12(1):1176. doi: 10.1038/s41597-025-05557-w.
Transcription readthrough occurs when RNA polymerase bypasses canonical termination sites, producing elongated RNA molecules called readthrough (RT) transcripts or downstream of gene (DoG) transcripts. Although RT transcripts have been implicated in stress responses and pathological states, their roles in healthy human tissues are poorly understood. This study collected and analyzed RT events across 43 healthy human tissues, identifying 75,248 RT events from 35,720 transcripts across 11,692 genes. The dataset encompasses the sequences, locations, expression profiles, and comprehensive annotation information of corresponding genes for RT transcripts. It provides a thorough elucidation of RT transcriptomics and its significance in gene regulation, offering a wealth of benchmark data to facilitate further research on RT transcripts.
当RNA聚合酶绕过典型终止位点时，就会发生转录通读，产生称为通读（RT）转录本或基因下游（DoG）转录本的延长RNA分子。尽管RT转录本与应激反应和病理状态有关，但其在健康人体组织中的作用却知之甚少。本研究收集并分析了43种健康人体组织中的RT事件，从11,692个基因的35,720个转录本中鉴定出75,248个RT事件。该数据集包含RT转录本对应基因的序列、位置、表达谱和全面注释信息。它全面阐释了RT转录组学及其在基因调控中的意义，提供了丰富的基准数据，以促进对RT转录本的进一步研究。
