Wang Yinxiao, Hao Yihe, Li Shengyan, Wen Ning, Yin Mingyuan, Lang Zhihong
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, 572000 China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
aBIOTECH. 2025 Mar 12;6(2):174-188. doi: 10.1007/s42994-025-00205-8. eCollection 2025 Jun.
In environmental biosafety assessments of glyphosate-tolerant crops, it is essential to evaluate the effects of cultivating these crops and applying glyphosate on the microbial community in the rhizosphere soil, which play a critical role in maintaining soil health, plant growth, and crop productivity. Maize () line GG2 was previously generated by transforming wild-type maize with the and genes, endowing GG2 with both active and passive resistance to glyphosate. However, the ecological risk of introducing these two new glyphosate-tolerance genes into maize, as well as glyphosate treatment, to rhizosphere microorganisms remain unclear. In this study, we used high-throughput sequencing to analyze the diversity and composition of the bacterial and fungal communities in the rhizosphere soil around biotech maize GG2, with (GG2-H) and without glyphosate treatment (GG2-N), compared with the near-isogenic, non-biotech maize line ZD958 at seven stages of growth. The structure and diversity of the bacterial and fungal communities of GG2-H were similar to those of ZD958, whereas glyphosate treatment had temporary effects on bacterial and fungal diversity and richness. The differences in the bacterial and fungal communities were associated with changes in soil properties such as pH, available phosphorus and organic matter, and seasonal changes. These factors, rather than maize lines, made the greatest contributions to the shifts in bacterial and fungal community structure. This study provides a comprehensive analysis of the effects of biotech crop cultivation, glyphosate treatment, soil physicochemical properties of soil, and maize growth stages on soil microbial communities, offering valuable insights for the large-scale adoption of biotech crops in China.
The online version contains supplementary material available at 10.1007/s42994-025-00205-8.
在耐草甘膦作物的环境生物安全评估中，评估种植这些作物和施用草甘膦对根际土壤微生物群落的影响至关重要，根际土壤微生物群落在维持土壤健康、植物生长和作物生产力方面发挥着关键作用。玉米（）品系GG2先前是通过用和基因转化野生型玉米而产生的，赋予GG2对草甘膦的主动和被动抗性。然而，将这两个新的耐草甘膦基因引入玉米以及草甘膦处理对根际微生物的生态风险仍不清楚。在本研究中，我们使用高通量测序分析了转基因玉米GG2周围根际土壤中细菌和真菌群落的多样性和组成，其中一组进行了草甘膦处理（GG2-H），另一组未进行草甘膦处理（GG2-N），并与近等基因非转基因玉米品系ZD958在七个生长阶段进行了比较。GG2-H的细菌和真菌群落结构和多样性与ZD958相似，而草甘膦处理对细菌和真菌的多样性和丰富度有暂时影响。细菌和真菌群落的差异与土壤性质如pH值、有效磷和有机质的变化以及季节变化有关。这些因素而非玉米品系对细菌和真菌群落结构的变化贡献最大。本研究全面分析了转基因作物种植、草甘膦处理、土壤理化性质和玉米生长阶段对土壤微生物群落的影响，为中国大规模采用转基因作物提供了有价值的见解。
在线版本包含可在10.1007/s42994-025-00205-8获取的补充材料。
