Yu Wenjie, Tian Haoran, Chen Yifan, Lyu Shanhua, Fan Yinglun
College of Agriculture and Biology, Liaocheng University, Liaocheng, China.
Front Plant Sci. 2025 Jun 26;16:1594197. doi: 10.3389/fpls.2025.1594197. eCollection 2025.
mediated genetic transformation has become an important method to study gene function and create new germplasm in plants. The classic genetic transformation process is time-consuming and laborious. In nature, some plants can propagate through detached leaves, which shows that the detached leaves can complete the dedifferentiation of leaf cell and bud differentiation through the intracellular hormone regulation of detached leaves without external hormones. Here, we report a simple leaf-cutting transformation (LCT) method without aseptic operation using mediated genetic transformation with detached leaves to complete the transgenic operation. Jonquil (), an ornamental plant with leaf propagation ability, was selected to transform via the LCT method using strain EHA105 and K599. Without selection pressure, the visual reporter , a system for the biosynthesis of betalains, was used in the LCT method. New colorful germplasms of Jonquil accumulated with betalains were obtained. The transgenic Jonquils transformed by EHA105 grow normally, but these transgenic Jonquils transformed by K599 have abnormal growth, manifested as dwarf phenotype, and most Jonquils had protrusions like tentacles or polyps on their leaves. Stable transformation in Jonquil should be mediated by , not
介导的遗传转化已成为研究植物基因功能和创造新种质的重要方法。经典的遗传转化过程耗时费力。在自然界中，一些植物可以通过离体叶片繁殖，这表明离体叶片可以在无外源激素的情况下，通过叶片细胞内激素调节完成叶细胞去分化和芽分化。在此，我们报道一种简单的无需无菌操作的切叶转化（LCT）方法，利用介导的离体叶片遗传转化完成转基因操作。选用具有叶片繁殖能力的观赏植物水仙（），使用菌株EHA105和K599通过LCT方法进行转化。在无选择压力的情况下，在LCT方法中使用了视觉报告基因，一种用于甜菜碱生物合成的系统。获得了积累甜菜碱的水仙新的彩色种质。用EHA105转化的转基因水仙生长正常，但用K599转化的这些转基因水仙生长异常，表现为矮化表型，且大多数水仙叶片上有类似触手或息肉的突起。水仙中的稳定转化应由介导，而不是
