Houssein I, Wilcox H, Barron J
Clin Chem. 1985 Dec;31(12):2028-30.
Incubation of serum and plasma samples at 56 degrees C for 30 min inactivates the HTLV-III virus. We assessed the effect of this procedure on results of routine biochemical tests by dividing samples of serum and plasma into two, heat-treating one while the other remained at room temperature. Samples were then analyzed in an SMA 16/60, an Astra-8, an Analox glucose analyzer, a Cobas Bio centrifugal analyzer, and manually for salicylate and acetaminophen (paracetamol). Most of the differences produced by heat treatment were not clinically significant, although heated samples proved unsuitable for use in assay of some commonly measured enzymes. Serum evidently is preferable to plasma for this procedure, and heat-treated serum samples can validly be used for most routine analyses. Thus this procedure makes safer the analysis of samples from patients with suspected or proven acquired immune deficiency syndrome (AIDS).
将血清和血浆样本在56摄氏度下孵育30分钟可使HTLV - III病毒失活。我们通过将血清和血浆样本分成两份，一份进行热处理，另一份保持在室温，来评估该程序对常规生化检测结果的影响。然后将样本在SMA 16/60、Astra - 8、Analox葡萄糖分析仪、Cobas Bio离心分析仪中进行分析，并手动检测水杨酸盐和对乙酰氨基酚（扑热息痛）。尽管经热处理的样本被证明不适用于某些常用酶的检测，但热处理产生的大多数差异在临床上并不显著。对于该程序，血清显然比血浆更可取，并且经热处理的血清样本可有效地用于大多数常规分析。因此，该程序使对疑似或已确诊获得性免疫缺陷综合征（艾滋病）患者的样本分析更安全。
