来自西班牙的新种（线虫纲：垫刃科）的形态学和分子特征

Morphological and molecular characterisation of sp. nov. (Nematoda: Tylenchidae) from Spain.

作者信息

Salazar-García R, Palomares-Rius J E, Cantalapiedra-Navarrete C, Liébanas G, Castillo P, Archidona-Yuste A

机构信息

https://ror.org/039vw4178Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Department of Crop Protection, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Spain.

Doctoral Programme in Agricultural, Food, Forestry Engineering and Sustainable Rural Development, at the University of Córdoba and the University of Seville, Spain.

出版信息

J Helminthol. 2025 Jul 11;99:e74. doi: 10.1017/S0022149X25100497.

DOI:10.1017/S0022149X25100497

PMID:40643005

Abstract

During nematode surveys conducted to investigate the biodiversity of plant-parasitic nematodes in Mediterranean olive groves with different management strategies (organic and conventional), a nematode population of the genus was detected in southern Spain. Application of integrative taxonomical approaches clearly demonstrated that it is a new species described herein as sp. nov., also representing the first report of the genus in Spain. The new species is amphimictic, characterised by a short body (563-774 μm); cuticle widely annulated (2.5-3.0 μm); total number of body annuli 214-226; 16 longitudinal ridges giving a tessellate body surface; stylet without distinct basal knobs (9.0-11.0 μm); and tail elongate-conoid, with tip bluntly rounded. The results of molecular analysis of D2-D3 28S rRNA, ITS rRNA, partial 18S rRNA, and cytochrome oxidase c subunit 1 (COI) gene sequences support for the new species status and clearly separated from and other species within Phylogenetic analyses of ribosomal and mitochondrial markers of this study suggested that is a monophyletic genus, clearly separated from

摘要

在对采用不同管理策略（有机和常规）的地中海橄榄园中的植物寄生线虫生物多样性进行调查时，在西班牙南部检测到了一个线虫属种群。综合分类学方法的应用清楚地表明，它是一个在此描述为新物种的新种，也是该属在西班牙的首次报道。该新种为两性融合，其特征为身体短小（563 - 774微米）；角质层有广泛的环纹（2.5 - 3.0微米）；身体环纹总数为214 - 226条；16条纵向脊使体表呈棋盘状；口针无明显基部瘤（9.0 - 11.0微米）；尾部细长呈锥形，末端钝圆。对D2 - D3 28S rRNA、ITS rRNA、部分18S rRNA和细胞色素氧化酶c亚基1（COI）基因序列的分子分析结果支持该新物种的地位，并与其他物种明显区分开来。本研究对核糖体和线粒体标记的系统发育分析表明，该属是一个单系属，与其他属明显分开。