Tian Huiling, Fei Gangxiang, Guan Jingqiang, Xu Zhongshun, Qu Jiaojiao, Dai Yongdong, Zou Xiao
Institute of Fungus Resources, College of Life Sciences, Guizhou University, Guiyang, Guizhou, China.
College of Tea Sciences, Guizhou University, Guiyang, Guizhou, China.
Microbiol Spectr. 2025 Aug 5;13(8):e0215924. doi: 10.1128/spectrum.02159-24. Epub 2025 Jul 11.
holds significant importance in forest arthropod population regulation, biological control of agricultural pests, and the development of bioactive substances. Investigating the microbial community composition of wild is significant for understanding its individual development process, understory cultivation, and taxonomy. In this study, a novel insect pathogenic fungus, , parasitizing Hymenoptera larvae, originating from Liaoning Province, China, was delineated based on morphology and phylogenetics. The microbial communities in the sclerotium and host surface complex of its different reproductive stages (sexual and asexual) were investigated. was detected within the subclade, being closely related to and . It was morphologically characterized by solitary or multiple stromata, semi-submerged perithecia, and viscous basidiospores. Proteobacteria, Actinobacteriota, and Bacteroidota were the major bacterial taxa, and Basidiomycota and Ascomycota were the major fungal taxa. , , and were abundant in the host surface complex that belongs to mycorrhizal fungi. The microbial co-occurrence network suggested that microbial relationships were predominantly positive correlations, and positive correlations were more pronounced in the asexual stage than the sexual stage. The microbial co-occurrence network of was more complex during the asexual stage; it exhibited greater metabolic activity during the asexual stage. These results have enhanced our understanding of the composition, diversity, and functions of the endophytic microbial community of , furnishing additional evidence for classifying its sexual and asexual stages and establishing a theoretical basis for subsequent research on its individual development.
exhibits two distinct life stages (asexual and sexual) in its natural environment. The symbiotic microorganisms associated with the fungus play a crucial role in its growth and development. We have identified a new species, , which belongs to the subclade. A large number of mycorrhizal fungi were found in the insect appendages of , whereas the fungal community within the sclerotium was predominantly composed of Ascomycota. During the asexual stage, exhibited greater microbial diversity and stronger positive correlations among microorganisms. Additionally, it possesses a richer array of metabolic pathways. These results have deepened our knowledge of the composition, diversity, and roles of the microbial community in , providing further evidence for distinguishing its sexual and asexual stages and laying a theoretical foundation for future research on its developmental processes.
在森林节肢动物种群调节、农业害虫生物防治以及生物活性物质开发中具有重要意义。研究野生[昆虫名称]的微生物群落组成对于了解其个体发育过程、林下栽培和分类学具有重要意义。在本研究中，基于形态学和系统发育学，描述了一种来自中国辽宁省、寄生于膜翅目幼虫的新型昆虫病原真菌[真菌名称]。研究了其不同生殖阶段（有性和无性）的菌核和宿主表面复合体中的微生物群落。[真菌名称]在[亚分支名称]亚分支中被检测到，与[相关真菌1]和[相关真菌2]密切相关。其形态特征为单个或多个子座、半埋生的子囊壳和粘性担孢子。变形菌门、放线菌门和拟杆菌门是主要的细菌类群，担子菌门和子囊菌门是主要的真菌类群。[菌根真菌1]、[菌根真菌2]和[菌根真菌3]在属于菌根真菌的宿主表面复合体中含量丰富。微生物共现网络表明微生物关系主要为正相关，且正相关在无性阶段比有性阶段更明显。[真菌名称]的微生物共现网络在无性阶段更复杂；它在无性阶段表现出更大的代谢活性。这些结果增强了我们对[真菌名称]内生微生物群落组成、多样性和功能的理解，为其有性和无性阶段的分类提供了额外证据，并为其个体发育的后续研究奠定了理论基础。
[真菌名称]在自然环境中表现出两个不同的生命阶段（无性和有性）。与该真菌相关的共生微生物在其生长和发育中起关键作用。我们鉴定了一个新物种[真菌名称]，它属于[亚分支名称]亚分支。在[昆虫名称]的昆虫附肢中发现了大量菌根真菌，而菌核内的真菌群落主要由子囊菌门组成。在无性阶段，[真菌名称]表现出更大的微生物多样性和微生物之间更强的正相关。此外，它拥有更丰富的代谢途径。这些结果加深了我们对[真菌名称]中微生物群落组成、多样性和作用的认识，为区分其有性和无性阶段提供了进一步证据，并为其发育过程的未来研究奠定了理论基础。
