Sclavons M M, Cordonnier C M, Mailleux P M, Heller F R, Desager J P, Harvengt C M
Clin Chim Acta. 1985 Dec 13;153(2):125-35. doi: 10.1016/0009-8981(85)90163-9.
An ultracentrifugation technique for isolation of the various lipoprotein fractions using a vertical rotor is described. By the use of a multiple discontinuous density gradient, very low density lipoproteins, low density lipoproteins and high density lipoproteins are sharply separated, without contamination of other lipoproteins or albumin. Centrifugation time is 80 min. The densities, electrophoretic mobilities, electron microscopic appearance and chemical composition are those of the expected classes of lipoproteins. Two different gradients are used to enhance the separation of very low density lipoproteins from low density lipoproteins on one hand and high density lipoproteins from infranatant on the other.
本文描述了一种使用垂直转子分离各种脂蛋白组分的超速离心技术。通过使用多重不连续密度梯度，极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白能被清晰分离，且无其他脂蛋白或白蛋白的污染。离心时间为80分钟。其密度、电泳迁移率、电子显微镜外观及化学组成均符合预期的脂蛋白类别。一方面，使用两种不同的梯度来增强极低密度脂蛋白与低密度脂蛋白的分离；另一方面，增强高密度脂蛋白与下层清液的分离。
