Chen Ling, Hu Jialian, Dai Ning, Du Pengfei, Tian Ye, Zhang Qianyi, Yang Chenghuai, Fu Bofan, Feng Saixiang, Liao Ming
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Division of Culture Collection and Testing, China Institute of Veterinary Drug Control, Beijing 100081, China.
Avian Dis. 2025 Jun;69(2):170-176. doi: 10.1637/aviandiseases-D-24-00042.
is the causative pathogen of infectious coryza. Isolates of obtained from China between 2008 and 2022 were characterized using 16S rRNA, , multilocus sequence typing, and biochemical tests to assess their genetic and biochemical diversity. All field isolates belonged to the same phylogenetic cluster together based on their 16S rRNA sequences. The similarity of the 16S rRNA sequences between the Chinese field isolates and type strain NCTC11296 was 96.5%-97.0%, and that among the Chinese field isolates was 99.7%-100%. Twenty typeable field isolates were categorized into A-1, B-1, and C-4 clades according to their sequences, but positioned in separate subclades alongside reference strains within each clade. Variable biochemical characteristics were observed between the type strain NCTC11296 and field isolates, with most Chinese field isolates utilizing inositol, L-fucose, arabitol, raffinose, and -acetyl neuraminic acid, unlike the type strain. The type strain NCTC11296 could produce acid from dextrin and maltose, unlike the 24 Chinese field isolates. Among the 36 isolates tested, one new allele and five new sequence types (STs) were identified; the most prevalent ST for Chinese field isolates was ST1 ( = 19; 79.2%). These results suggest that the Chinese field isolates of were genetically and biochemically different from the type strain.
是传染性鼻炎的致病病原体。对2008年至2022年期间从中国分离得到的菌株，使用16S rRNA、[此处原文缺失相关内容]、多位点序列分型和生化试验进行特征分析，以评估其遗传和生化多样性。根据16S rRNA序列，所有田间分离株共同属于同一个系统发育簇。中国田间分离株与模式菌株NCTC11296之间的16S rRNA序列相似性为96.5%-97.0%，中国田间分离株之间的相似性为99.7%-100%。根据[此处原文缺失相关内容]序列，20株可分型的田间分离株被归类为A-1、B-1和C-4分支，但在每个分支内与参考菌株位于不同的亚分支中。在模式菌株NCTC11296和田间分离株之间观察到可变的生化特征，与模式菌株不同，大多数中国田间分离株利用肌醇、L-岩藻糖、阿拉伯糖醇、棉子糖和N-乙酰神经氨酸。与24株中国田间分离株不同，模式菌株NCTC11296可从糊精和麦芽糖中产酸。在测试的36株分离株中，鉴定出1个新的[此处原文缺失相关内容]等位基因和5种新的序列类型（STs）；中国田间分离株中最常见的ST是ST1（n = 19；79.2%）。这些结果表明，中国田间分离的[此处原文缺失相关内容]菌株在遗传和生化方面与模式菌株不同。
