Mousavi-Ejarestaghi Negin, Shaabani Alireza, Milani Shahriyar, Motedayen Maral, Maghsoudian Samane, Fatahi Yousef, Razavi Malihe Sadat, Khanavi Mahnaz, Mousavi-Ejarestaghi Negar, Ramezani Mohamadmehdi, Sayyari Nastaran, Motasadizadeh Hamidreza, Javar Hamid Akbari
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Polymer and Materials Chemistry, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, Tehran, Iran.
Int J Biol Macromol. 2025 Aug;320(Pt 3):145826. doi: 10.1016/j.ijbiomac.2025.145826. Epub 2025 Jul 9.
Burns are severe injuries with significant medical and socio-economic impacts. For this purpose, a three-layer pH-responsive nanofibrous wound dressing was developed for burn treatment using the electrospinning method. The dressing consists of an external layer of polycaprolactone (PCL) nanofibers, a middle layer of hybrid PCL nanofibers and Hamamelis virginiana (HV) extract in chitosan/polyvinyl alcohol (CS/PVA) nanofibers, and an internal layer of hybrid PCL nanofibers and Diclofenac Sodium (DF) in CS/PVA nanofibers. The dressing was characterized through in vitro and in vivo analysis. The addition of DF and HV extract increased the nanofiber diameters to 170 nm and 157 nm, respectively. DF release was 63.8 % at alkaline pH, 73.7 % at neutral pH and 83.5 % at acidic pH, while HV release was 56.1 % at alkaline pH, 64 % at neutral pH and 87 % at acidic pH. All formulations maintained ≥85 % cell viability with low cellular adhesion and controlled release, suggesting potential for wound healing. Ultimately, in vivo experiments showed that HV/DF-loaded nanofibers achieved ∼98 % wound closure within 21 days, demonstrating superior healing in both visual and histological evaluations. Overall, the results suggest HV/DF-loaded nanofibers formulation as a potential treatment for burn wounds.
烧伤是具有重大医学和社会经济影响的严重损伤。为此，采用静电纺丝法制备了一种用于烧伤治疗的三层pH响应性纳米纤维伤口敷料。该敷料由外层聚己内酯（PCL）纳米纤维、中间层壳聚糖/聚乙烯醇（CS/PVA）纳米纤维中混合的PCL纳米纤维和北美金缕梅（HV）提取物以及内层壳聚糖/聚乙烯醇（CS/PVA）纳米纤维中混合的PCL纳米纤维和双氯芬酸钠（DF）组成。通过体外和体内分析对该敷料进行了表征。添加DF和HV提取物后，纳米纤维直径分别增加到170nm和157nm。DF在碱性pH下的释放率为63.8%，在中性pH下为73.7%，在酸性pH下为83.5%，而HV在碱性pH下的释放率为56.1%，在中性pH下为64%，在酸性pH下为87%。所有配方均保持≥85%的细胞活力，细胞粘附性低且具有控释性能，表明具有伤口愈合潜力。最终，体内实验表明，负载HV/DF的纳米纤维在21天内实现了约98%的伤口闭合，在视觉和组织学评估中均显示出优异的愈合效果。总体而言，结果表明负载HV/DF的纳米纤维配方是治疗烧伤创面的一种潜在方法。
