Zhang Kai, Chen Jianchao, Duan Binwei, You Canbei, Zhou Wenchuan, Zhao Yanfei, Yang Shaosen, Wu Jinhui, Shi Qiong
Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518057, China.
Shenzhen Ocean Development Promotion Center, Shenzhen, 518067, China.
Sci Data. 2025 Jul 11;12(1):1194. doi: 10.1038/s41597-025-05475-x.
Humphead wrasse, Cheilinus undulatus, is an endangered fish species with high economic and ecological value as well as natural sex change from female to male, while sexual selection occurs in breeding aggregations. In our present study, we constructed the first gap-free telomere-to-telomere (T2T) genome assembly for humphead wrasse, by integration of PacBio HiFi, ONT Ultra-long and Hi-C sequencing techniques. With 99% of the entire sequences anchored into 24 chromosomes, this haplotypic genome assembly spans approximately 1.25 Gb and presents a complete set of 48 telomeres and 24 centromeres. In terms of correctness (quality value QV: 53.447) and completeness (BUSCO score: 99.3%), this chromosome-scale assembly is indeed of high quality. We predicted 658.03 Mb of repetitive sequences and annotated 26,609 protein-coding genes in the assembled genome. This high-quality T2T genome assembly not only facilitates the genetic conservation of humphead wrasse, but also offers fundamental genomic data for supporting in-depth investigations on functional genomics, genetic diversity, and selective breeding for this economically important teleost.
波纹唇鱼（Cheilinus undulatus）是一种濒危鱼类，具有很高的经济和生态价值，且具有从雌性向雄性的自然性转变，同时在繁殖群体中存在性选择。在我们目前的研究中，我们通过整合PacBio HiFi、ONT超长读长和Hi-C测序技术，构建了首个波纹唇鱼的端粒到端粒无间隙（T2T）基因组组装。该单倍型基因组组装将99%的全序列锚定到24条染色体上，跨度约为1.25Gb，呈现出一套完整的48个端粒和24个着丝粒。从正确性（质量值QV：53.447）和完整性（BUSCO评分：99.3%）来看，这个染色体级别的组装确实具有很高的质量。我们在组装的基因组中预测了658.03Mb的重复序列，并注释了26,609个蛋白质编码基因。这个高质量的T2T基因组组装不仅有助于波纹唇鱼的遗传保护，还为支持对这种具有重要经济价值的硬骨鱼进行功能基因组学、遗传多样性和选择性育种的深入研究提供了基础基因组数据。
