Li Jiawei, A Yingjie, Liu Minghao, Li Xin, Zhan Yilin, Khashi U Rahman Muhammad, Zhou Xingang
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region, Ministry of Agriculture and Rural Affairs), Heilongjiang Key Laboratory of Cold Area Protected Horticulture, Department of Horticulture, Northeast Agricultural University, Harbin, 150030, China.
School for the Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ, 85287, USA.
Curr Microbiol. 2025 Jul 12;82(9):382. doi: 10.1007/s00284-025-04278-y.
Extracellular DNA (eDNA), as a potential plant autotoxin, can cause soil sickness, negatively affecting plant growth and reducing crop yield. Some microbial taxa can degrade eDNA; however, the effect of these bacteria on plant growth and rhizosphere microbial communities is unknown. In this study, eDNA-degrading bacterial strains were screened from soils, and their eDNA-degrading ability and plant-growth-promoting characteristics were tested in vitro. The effects of the most potent eDNA-degrading strain on tomato plant growth were assessed, and changes in the rhizosphere bacterial community were analyzed using high-throughput amplicon sequencing. Among a total of more than 600 isolated strains, Pseudomonas sp. F204 showed the strongest eDNA-degradation capacity and exhibited phosphate solubilization, siderophore, and indole-3-acetic acid production abilities. Pot experiment showed that inoculation of Pseudomonas sp. F204 significantly altered tomato rhizosphere bacterial community structure and composition, and stimulated the growth of tomato seedlings. Overall, this study shows that eDNA-degrading bacteria can alter rhizosphere bacterial community and promote plant growth, and highlights the important role of soil microbial community in alleviating autotoxicity in continuous cropping systems.
细胞外DNA（eDNA）作为一种潜在的植物自毒物质，可导致土壤病害，对植物生长产生负面影响并降低作物产量。一些微生物类群能够降解eDNA；然而，这些细菌对植物生长和根际微生物群落的影响尚不清楚。在本研究中，从土壤中筛选出eDNA降解细菌菌株，并在体外测试了它们的eDNA降解能力和促进植物生长的特性。评估了最具活性的eDNA降解菌株对番茄植株生长的影响，并使用高通量扩增子测序分析了根际细菌群落的变化。在总共600多个分离菌株中，假单胞菌属F204表现出最强的eDNA降解能力，并具有溶解磷酸盐、产生铁载体和吲哚-3-乙酸的能力。盆栽试验表明，接种假单胞菌属F204显著改变了番茄根际细菌群落结构和组成，并促进了番茄幼苗的生长。总体而言，本研究表明，eDNA降解细菌可改变根际细菌群落并促进植物生长，并突出了土壤微生物群落在缓解连作系统自毒作用中的重要作用。
