Bugno-Poniewierska Monika, Bielecka Monika, Pietras Natalia, Kij-Mitka Barbara, Podstawski Zenon, Długosz Bogusława
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, al. Mickiewicza 24/28, 30-059 Krakow, Poland.
Animals (Basel). 2025 Jun 28;15(13):1915. doi: 10.3390/ani15131915.
The Hucul horse is a Polish primitive breed with a small population size, which highlights the importance of preserving the genetic resources. The cryopreservation of semen is essential for creating gene banks, but its effect on the acrosome reaction in Hucul stallions has not yet been investigated. The acrosome reaction is one of the most important physiological events associated with the fertilization process. Therefore, our goal was to determine the level of acrosome reaction in chilled and frozen/thawed Hucul stallion semen using the FluoAcro test and the SCA semen analysis system. We found that semen cryopreservation significantly reduced sperm motility and was associated with an increased percentage of acrosome-reacted spermatozoa. It should be noted, however, that, in this case, there was no negative control, and the results may reflect acrosomal damage rather than the elicited responses. Further validation of the methods with equine sperm and the inclusion of a control are recommended.
胡库勒马是一种波兰原始品种，种群规模较小，这凸显了保护其遗传资源的重要性。精液冷冻保存对于建立基因库至关重要，但其对胡库勒种公马顶体反应的影响尚未得到研究。顶体反应是与受精过程相关的最重要生理事件之一。因此，我们的目标是使用FluoAcro试验和SCA精液分析系统，确定冷藏和冷冻/解冻后的胡库勒种公马精液中的顶体反应水平。我们发现精液冷冻保存显著降低了精子活力，并与顶体反应精子的百分比增加有关。然而，应该指出的是，在这种情况下，没有阴性对照，结果可能反映的是顶体损伤而非引发的反应。建议使用马精子对这些方法进行进一步验证并纳入对照。
