Longshaw Matt, Quest Bradley, Miller Walt, Oba Patricia M, Swanson Olivia R, Swanson Kelly S, Miller Kathryn
Calysta (UK), Ltd., Wilton Centre, Redcar TS10 4RF, UK.
BSM Partners, 83 W Champions Boulevard, Rogers, AR 72712, USA.
Animals (Basel). 2025 Jul 4;15(13):1975. doi: 10.3390/ani15131975.
Thirty-two healthy adult dogs (16 males and 16 females) were fed control kibble diets for one month, followed by six months (Weeks 0 to 25) of diets containing either 0, 4, 6, or 8% cultured protein derived from (FeedKind Pet, FK), then they were fed control diets (0% FK) for a further two months (Weeks 25 to 34). The diets were isonitrogenous, isolipidic, and isocaloric and stage- and age-specific. The dogs were assessed for overall health, weight gain, and body condition score (BCS). Blood samples were collected 1 week prior to randomization, during acclimation, then in Weeks 5, 13, 25, 30, 32, and 34 for hematology, coagulation, and clinical chemistry; urine was collected according to the same time schedule for urinalysis. Feces were assessed for parasite load and presence of occult blood during Weeks 5, 9, 13, 17, 21, and 25. Fecal samples were collected during acclimation and Weeks 25 and 34 for fecal microbiome analysis and in Week 25 for apparent total gastrointestinal tract digestibility (ATTD). All dogs maintained a healthy weight and BCS throughout the study. Hematology parameters were within normal limits at the end of each phase of the study. With the exception of a decrease in serum phosphorus level and in urine pH in all groups at the end of the study, urine and serum chemistry results were within normal limits at the end of each phase. ATTD values for organic matter, protein, and energy exceeded 80%, whilst digestibility values for copper were around 20%. The fecal microbiome was dominated by Firmicutes. Alpha diversity increased during the safety phase before returning to baseline levels during the washout phase. The dominant genera in all groups were , , , , , , and . The study has shown that the inclusion of cultured protein at up to 8% of the total diet of adult dogs can provide sufficient nutrition and is safe with no long-term effects on a range of health parameters.
32只健康成年犬（16只雄性和16只雌性）先喂食对照干粮饮食1个月，随后6个月（第0至25周）喂食含有0%、4%、6%或8%源自[未提及具体来源]的培养蛋白（FeedKind Pet，FK）的饮食，然后再喂食对照饮食（0% FK）2个月（第25至34周）。这些饮食是等氮、等脂和等热量的，且根据阶段和年龄定制。对犬只的整体健康、体重增加和身体状况评分（BCS）进行评估。在随机分组前1周、适应期、然后在第5、13、25、30、32和34周采集血样进行血液学、凝血和临床化学检测；尿液按照相同时间表采集进行尿液分析。在第5、9、13、17、21和25周评估粪便中的寄生虫负荷和潜血情况。在适应期、第25和34周采集粪便样本进行粪便微生物群分析，并在第25周进行表观全胃肠道消化率（ATTD）检测。在整个研究过程中，所有犬只均保持健康体重和BCS。在研究各阶段结束时，血液学参数均在正常范围内。除研究结束时所有组血清磷水平和尿液pH值下降外，各阶段结束时尿液和血清化学结果均在正常范围内。有机物、蛋白质和能量的ATTD值超过80%，而铜的消化率值约为20%。粪便微生物群以厚壁菌门为主。α多样性在安全阶段增加，然后在洗脱阶段恢复到基线水平。所有组中的优势菌属为[未提及具体菌属]、[未提及具体菌属]、[未提及具体菌属]、[未提及具体菌属]、[未提及具体菌属]、[未提及具体菌属]和[未提及具体菌属]。该研究表明，在成年犬总饮食中添加高达8%的培养蛋白可提供充足营养且安全，对一系列健康参数无长期影响。
