Balouei Fatemeh, Stefanon Bruno, Armone Rosangela, Randazzo Andrea, Chiofalo Biagina
Department of Agrifood, Environmental and Animal Science, University of Udine, Via delle Scienze 206, 33100 Udine, Italy.
Department of Veterinary Sciences, University of Messina, Via Palatucci Snc, 98168 Messina, Italy.
Microorganisms. 2025 Jan 9;13(1):121. doi: 10.3390/microorganisms13010121.
Two extruded diets isoenergetic, isonitrogenous, and isolipidic were formulated with poultry meal (control diet) as the source of animal-origin proteins (160 g/kg of feed) or with 90 g/kg of poultry meal and 70 g/kg of hydrolyzed feather meal (treated diet) and were fed to eight dogs (four adult female and four adult male English Setters). Body condition, muscle condition, and fecal consistency scores and body weight were monitored at the beginning of the trial and after 3, 7, 15, and 45 days, and no significant differences ( > 0.05) were observed between diets and between sex. Fecal samples, collected at the same time points, were analyzed for microbiota composition. No significant difference was calculated for the alpha diversity index between control diet and treated diet, nor for the diets × times of sampling interaction and for sex. Beta diversity was different (-value 0.001) between the control and treated groups. The beta diversity between sexes was significantly different (-value = 0.047). Linear Discriminant analysis effect size analysis revealed significant differences between dietary groups, identifying , , , , , and as more abundant in the CTR diet, while , , , , , and were more abundant in the treated diet. Sex differences were also significant, with 25 taxa differing between male and female dogs. Overall, the study underscores the impact of HFM and sex on gut microbiota composition in dogs, with potential implications for dietary interventions and microbiome research.
配制了两种等能量、等氮和等脂的挤压日粮，一种以禽肉粉（对照日粮）作为动物源蛋白质来源（每千克饲料含160克），另一种以90克/千克禽肉粉和70克/千克水解羽毛粉（处理日粮），并将其喂给8只狗（4只成年雌性和4只成年雄性英国塞特犬）。在试验开始时以及3天、7天、15天和45天后监测身体状况、肌肉状况、粪便稠度评分和体重，日粮之间以及性别之间均未观察到显著差异（>0.05）。在相同时间点采集粪便样本，分析微生物群组成。对照日粮和处理日粮之间的α多样性指数、日粮×采样时间交互作用以及性别之间均未计算出显著差异。对照组和处理组之间的β多样性不同（P值0.001）。性别之间的β多样性也有显著差异（P值=0.047）。线性判别分析效应大小分析显示日粮组之间存在显著差异，确定在对照日粮中 、 、 、 、 、 更为丰富，而在处理日粮中 、 、 、 、 、 更为丰富。性别差异也很显著，雄性和雌性狗之间有25个分类群不同。总体而言，该研究强调了水解羽毛粉和性别对狗肠道微生物群组成的影响，对饮食干预和微生物组研究具有潜在意义。
