Tříska Jan, Vrchotová Naděžda, Strohalm Jan, Houška Milan, Kováříková Eliška, Novotná Pavla, Bednář Jan, Pavela Roman
Global Change Research Institute, CAS, Bělidla 986/4a, 603 00 Brno, Czech Republic.
Faculty of Agriculture and Technology, University of South Bohemia, Studentská 1668, 370 05 České Budějovice, Czech Republic.
Foods. 2025 Jun 21;14(13):2165. doi: 10.3390/foods14132165.
The paper deals with the investigation of the ascorbic acid influence on the analytical results of polyphenol content in the samples of the spearmint, fennel, and thyme homogenates. The homogenates without and with addition of ascorbic acid and water were prepared and stabilized by high-pressure treatment. Their analysis was accomplished by high-performance liquid chromatography (HPLC) with DAD detection and by combination of liquid chromatography with mass spectrometry (LC-MS). Volatile terpenes were analyzed in all homogenates by combination of gas chromatography with mass spectrometry technique (GC-MS). The content of polyphenols of acidic nature, e.g., rosmarinic acid, revealed the highest difference between analytical results of the samples with and without ascorbic acid. Finally, prepared herb homogenates are suitable food supplements, which will find increasing application in various food preparations.
本文研究了抗坏血酸对留兰香、茴香和百里香匀浆样品中多酚含量分析结果的影响。制备了添加和未添加抗坏血酸及水的匀浆，并通过高压处理使其稳定。采用带二极管阵列检测的高效液相色谱法（HPLC）以及液相色谱-质谱联用（LC-MS）对其进行分析。通过气相色谱-质谱联用技术（GC-MS）对所有匀浆中的挥发性萜类进行分析。酸性多酚（如迷迭香酸）的含量在添加和未添加抗坏血酸的样品分析结果之间显示出最大差异。最后，制备的草本匀浆是合适的食品补充剂，将在各种食品制剂中得到越来越广泛的应用。
