Polak Paweł, Radzki Radosław P, Bieńko Marek, Szymańczyk Sylwia, Topolska Kinga, Manastyrska-Stolarczyk Małgorzata, Szponar Jarosław
Department of Orthopedics and Traumatology, Provincial Specialist Hospital in Biała Podlaska, ul. Terebelska 57-65, 21-500 Biała Podlaska, Poland.
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland.
Nutrients. 2025 Jun 25;17(13):2110. doi: 10.3390/nu17132110.
: This study aimed to establish the potential osteotropic effect of pine pollen on bone metabolism in male rats during the development of osteopenia induced by orchidectomy (ORX). We also established the effect of gonadectomy and pine pollen on the characteristics of calf muscles. : This study was conducted using 40 male Wistar rats divided into one sham-operated (SHO) and four ORX groups. The SHO rats and one ORX group (negative control) were treated with physiological saline (PhS). The remaining ORX groups received exclusively testosterone (positive control) and two doses of pine pollen (50 and 150 mg/kg b.w.), respectively. The rats were killed 60 days later and their right tibia and left pelvic limbs were isolated. The tibia was analyzed using densitometry, computed tomography, and a bending machine to determine densitometry, structure, and mechanical properties, respectively. The left pelvic limb allowed for measurements of area, density, and fat tissue in the calf muscle. : The dose of 150 mg/kg b.w. inhibited the development of atrophic changes, both in the cortical and trabecular bone tissue. The dose of 50 mg/kg b.w. also has a protective effect on bones but is less pronounced and concerns only the trabecular bone tissue. The higher dose of pine pollen inhibited the catabolism of the calf muscles by maintaining the density and surface area as in the SHO group. It also limited the accumulation of intramuscular and subcutaneous adipose tissue. : It is worth emphasizing the osteoprotective effectiveness of pine pollen, especially when administered in larger doses, which demonstrates the possibility of its use in the prevention of the development of osteoporosis in males.
本研究旨在确定松花粉对去势（ORX）诱导的雄性大鼠骨质疏松症发展过程中骨代谢的潜在促骨作用。我们还确定了去势和松花粉对小腿肌肉特征的影响。本研究使用40只雄性Wistar大鼠，分为1个假手术组（SHO）和4个ORX组。SHO组大鼠和1个ORX组（阴性对照组）用生理盐水（PhS）处理。其余ORX组分别单独接受睾酮（阳性对照组）和两剂松花粉（50和150mg/kg体重）。60天后处死大鼠，分离其右胫骨和左骨盆肢体。分别使用骨密度测定法、计算机断层扫描和弯曲试验机对胫骨进行分析，以确定骨密度、结构和力学性能。左骨盆肢体用于测量小腿肌肉的面积、密度和脂肪组织。150mg/kg体重的剂量抑制了皮质骨和小梁骨组织萎缩性变化的发展。50mg/kg体重的剂量对骨骼也有保护作用，但不太明显，且仅涉及小梁骨组织。较高剂量的松花粉通过维持与SHO组相同的密度和表面积来抑制小腿肌肉的分解代谢。它还限制了肌肉内和皮下脂肪组织的积累。值得强调的是松花粉的骨保护有效性，尤其是大剂量给药时，这表明其有可能用于预防男性骨质疏松症的发展。
