Balcells-Esponera Carla, Aldecoa-Bilbao Victoria, Borràs-Novell Cristina, López-Abad Miriam, Valls Lafón Anna, Batllori Tragant Marta, Izquierdo Renau Montserrat, Del Rey Hurtado de Mendoza Beatriz, Herranz-Barbero Ana, Iglesias-Platas Isabel
Neonatology Department, BCNatal-Centre de Medicina Maternofetal i Neonatologia de Barcelona, Hospital Sant Joan de Déu, Universitat de Barcelona, 08950 Barcelona, Spain.
Neonatology Department, BCNatal-Centre de Medicina Maternofetal i Neonatologia de Barcelona, Hospital Clínic, Universitat de Barcelona, 08028 Barcelona, Spain.
Nutrients. 2025 Jun 30;17(13):2177. doi: 10.3390/nu17132177.
Calprotectin and intestinal fatty acid-binding protein (IFABP) may reflect the intestinal maturation process of very preterm infants (VPI) but have also been associated with gut inflammation. To establish normative values for fecal calprotectin (FC) and urinary intestinal fatty acid-binding protein (uIFABP) in VPI and to study their correlations with demographic and clinical factors. A cohort of VPI (born before or at 32.0 weeks of gestation) was recruited in two neonatal intensive care units. Urine and fecal samples were collected at 1, 4 and 8 weeks of life to measure urinary IFABP (normalized to creatinine as uIFABP/Cr) and FC, respectively. UIFABP was determined by ELISA and FC by fluoroenzyme immunoassay. : 194 newborns had at least one valid biomarker measurement. The study cohort mean gestational age was 28.9 ± 2.3 weeks and mean birth weight 1178 ± 365 g. Although uIFABP/Cr concentrations differed between the two centres, they were negatively correlated with gestational age, with a statistically significant correlation observed in both centres at week 4 (Hospital Clínic: Spearman's rho -0.500; = 0.000 and Hospital Sant Joan de Déu: Spearman's rho -0.474; = 0.000). Conversely, FC showed a positive significant correlation at the same time point (Spearman's rho 0.302; = 0.006). At week one, FC increased with antibiotic exposure (28 mcg/g of stool per antibiotic day, 95%CI 3-57; = 0.028). FC at week 4 was inversely correlated with mother's own milk (MOM) exposure during the first month (Spearman's rho -0.253; = 0.023). : uIFABP/Cr and FC are associated with gestational age at 4 weeks and FC is also influenced by antibiotic treatment and MOM exposure.
钙卫蛋白和肠脂肪酸结合蛋白（IFABP）可能反映极早产儿（VPI）的肠道成熟过程，但也与肠道炎症有关。为了确定VPI粪便钙卫蛋白（FC）和尿肠脂肪酸结合蛋白（uIFABP）的标准值，并研究它们与人口统计学和临床因素的相关性。在两个新生儿重症监护病房招募了一组VPI（出生于妊娠32.0周之前或之时）。在出生后1、4和8周收集尿液和粪便样本，分别测量尿IFABP（以肌酐标准化为uIFABP/Cr）和FC。UIFABP通过酶联免疫吸附测定法测定，FC通过荧光酶免疫测定法测定。194名新生儿至少有一次有效的生物标志物测量。研究队列的平均胎龄为28.9±2.3周，平均出生体重为1178±365克。尽管两个中心的uIFABP/Cr浓度不同，但它们与胎龄呈负相关，在第4周两个中心均观察到具有统计学意义的相关性（临床医院：斯皮尔曼相关系数-0.500；P = 0.000；圣琼德迪乌医院：斯皮尔曼相关系数-0.474；P = 0.000）。相反，FC在同一时间点显示出正相关且具有统计学意义（斯皮尔曼相关系数0.302；P = 0.006）。在第1周，FC随着抗生素暴露而增加（每使用一天抗生素，粪便中增加28微克/克，95%置信区间3-57；P = 0.028）。第4周的FC与第一个月内母亲自己的母乳（MOM）喂养呈负相关（斯皮尔曼相关系数-0.253；P = 0.023）。结论：uIFABP/Cr和FC与4周时的胎龄有关，FC也受抗生素治疗和MOM喂养的影响。
