ENHO在胰腺腺癌中的作用：一种生物信息学方法。

The Role of ENHO in Pancreatic Adenocarcinoma: A Bioinformatics Approach.

作者信息

Younis Osama M, Al-Sharif Zeid K, Saeed Ahmad E, Qubbaj Fares B, Yasin Jehad A, Nour Tasnim, Alami Idrissi Yassine, Saeed Anwaar

机构信息

Division of Hematology & Oncology, Department of Medicine, University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA 15232, USA.

School of Medicine, The University of Jordan, Amman 11942, Jordan.

出版信息

Cancers (Basel). 2025 Jun 25;17(13):2139. doi: 10.3390/cancers17132139.

DOI:10.3390/cancers17132139

PMID:40647442

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12248484/

Abstract

BACKGROUND

Pancreatic adenocarcinoma (PAAD) is an aggressive subtype of pancreatic cancer that is estimated to have a 5-year overall survival rate of only 13%. Most patients present with advanced disease with unpredictable outcomes. The identification of prognostic biomarkers is important to accurately stratify these patients.

METHODS

We investigated the molecular and survival-related role of in PAAD by analyzing TGCA mRNA and miRNA data. Survival analysis was conducted using TIMER2.0, "survival", and "survminer". Gene set enrichment analysis was conducted using enrichr, while miRNA-mRNA interactions were identified using "multiMiR". Immune infiltration was assessed using CIBERSORT ABS and ImmuCellAI.

RESULTS

We observed that was strikingly downregulated in PAAD tissues ( = 3.68 × 10), and patients with higher levels enjoyed significantly better overall survival (HR = 0.597; 95% CI: 0.419-0.852; < 0.01). Pathway analysis showed that genes co-upregulated with were enriched for insulin secretion and ion channel activity, whereas those co-downregulated were related to epithelial-mesenchymal transition and extracellular matrix remodeling. Higher also tracked with increased CD8 T-cell infiltration and correlated positively with PDCD1 and LAG3 but negatively with B7-H3, CD70, and NT5E.

CONCLUSIONS

Our results point to a protective role for in pancreatic adenocarcinoma.

摘要

背景

胰腺腺癌（PAAD）是胰腺癌的一种侵袭性亚型，据估计其5年总生存率仅为13%。大多数患者就诊时已处于晚期，预后难以预测。识别预后生物标志物对于准确分层这些患者很重要。

方法

我们通过分析TGCA mRNA和miRNA数据，研究了[具体基因名称未给出]在PAAD中的分子和生存相关作用。使用TIMER2.0、“survival”和“survminer”进行生存分析。使用enrichr进行基因集富集分析，同时使用“multiMiR”识别miRNA-mRNA相互作用。使用CIBERSORT ABS和ImmuCellAI评估免疫浸润。

结果

我们观察到[具体基因名称未给出]在PAAD组织中显著下调（= 3.68 × 10），[具体基因名称未给出]水平较高的患者总体生存率显著更好（HR = 0.597；95% CI：0.419 - 0.852；< 0.01）。通路分析表明，与[具体基因名称未给出]共同上调的基因在胰岛素分泌和离子通道活性方面富集，而共同下调的基因与上皮-间质转化和细胞外基质重塑有关。较高的[具体基因名称未给出]水平还与CD8 T细胞浸润增加相关，并且与PDCD1和LAG3呈正相关，但与B7-H3、CD70和NT5E呈负相关。