Karamalis Constantinos, Xirogianni Athanasia, Simantirakis Stelmos, Delegkou Marina, Papandreou Anastasia, Tzanakaki Georgina
National Meningitis Reference Laboratory, Department of Public Health Policy, School of Public Health, University of West Attica, 1152 Athens, Greece.
Diagnostics (Basel). 2025 Jun 26;15(13):1632. doi: 10.3390/diagnostics15131632.
: Meningitis due to the species is a severe central nervous system infection caused by various microorganisms belonging to spp. Its accurate identification is critical for effective clinical management. This study aimed to identify spp. causing meningitis in Greece over a nine-year period using PCR and sequencing-based methods. : A total of 189 clinical samples, collected between 2015 and 2024 from patients suffering from meningitis and/or septicemia, were analyzed by the use of a combination of multiplex polymerase chain reaction (PCR) assays and gene sequencing for further species identification. : Sample analysis identified 70 samples as (18.52%) (GAS) and (18.52%) (GBS), while 119 (62.96%) were recorded as non-typable spp. Further analysis using sequencing methods revealed that the most frequent spp. belonged to the mitis group (42.86%) and the pyogenic group (20.17%). A higher prevalence was observed in children aged 0-14 years old and adults over 50 years old. : This study highlights the use of molecular diagnostics in identifying other spp., providing insights into age-related susceptibility and epidemiological trends. Future studies are needed to explore the pathogenic role of the identified spp.
由该菌种引起的脑膜炎是一种严重的中枢神经系统感染，由属于该菌属的多种微生物引起。其准确鉴定对于有效的临床管理至关重要。本研究旨在使用基于聚合酶链反应（PCR）和测序的方法，鉴定希腊九年间引起脑膜炎的该菌属菌种。：共收集了189份2015年至2024年间从患有脑膜炎和/或败血症的患者身上采集的临床样本，通过多重聚合酶链反应（PCR）检测和基因测序相结合的方法进行分析，以进一步鉴定菌种。：样本分析确定70份样本为A群链球菌（18.52%）（GAS）和B群链球菌（18.52%）（GBS），而119份（62.96%）被记录为不可分型的该菌属菌种。使用测序方法的进一步分析显示，最常见的该菌属菌种属于缓症链球菌组（42.86%）和化脓性链球菌组（20.17%）。在0至14岁的儿童和50岁以上的成年人中观察到较高的患病率。：本研究强调了分子诊断在鉴定其他该菌属菌种方面的应用，为年龄相关易感性和流行病学趋势提供了见解。未来需要开展研究以探索已鉴定的该菌属菌种的致病作用。
