Gunasekara Sandani M, Mendis Chamara L, Perera Dinum, Wijayasinghe Malaka M
Department of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale 50300, Sri Lanka.
Faculty of Applied Sciences, Rajarata University of Sri Lanka, Mihintale 50300, Sri Lanka.
Plants (Basel). 2025 Jun 20;14(13):1893. doi: 10.3390/plants14131893.
Roxb., an economically and ecologically significant halophytic species native to Sri Lanka, produces dimorphic seeds. Despite their importance for commercial cultivation and conservation, germination studies of these dimorphic seeds remain limited. This study investigated the effects of temperature (25 °C, 30 °C, 35 °C), gibberellic acid (GA) treatment, geographic location of seed source (Jaffna vs. Puttalam coastal regions), seed type (central vs. lateral), and perianth presence/absence on germination under controlled conditions. Our results show that temperature, GA, and geographic location of the seed source significantly influenced seed germination. This study presents the first documented evidence of physiological dormancy (PD) in seeds, with successful dormancy breaking achieved using GA treatment at 25 °C. Although perianth and seed type alone had no significant direct effects on germination, they were involved in multiple significant interactions-two-, three-, and four-way-with other factors. These findings highlight the multifactorial regulation of seed germination, suggesting that tailored propagation strategies, incorporating environmental and physiological variables, can optimize germination. These findings offer practical solutions for enhancing germination in saline agriculture and habitat restoration efforts of .
罗克斯伯（Roxb.）是一种原产于斯里兰卡的具有经济和生态意义的盐生植物物种，产生二形种子。尽管它们对商业种植和保护很重要，但对这些二形种子的萌发研究仍然有限。本研究调查了温度（25℃、30℃、35℃）、赤霉素（GA）处理、种子来源的地理位置（贾夫纳与普塔勒姆沿海地区）、种子类型（中央与侧面）以及有无花被对控制条件下种子萌发的影响。我们的结果表明，温度、GA和种子来源的地理位置对种子萌发有显著影响。本研究首次记录了种子存在生理休眠（PD）的证据，在25℃下使用GA处理成功打破了休眠。虽然单独的花被和种子类型对萌发没有显著的直接影响，但它们与其他因素存在多种显著的相互作用——二因素、三因素和四因素相互作用。这些发现突出了种子萌发的多因素调控，表明结合环境和生理变量的定制繁殖策略可以优化萌发。这些发现为提高盐碱地农业中的萌发率和栖息地恢复工作提供了实际解决方案。
