Yang Xinhui, Liu Zisheng, Chen Jun, Zhang Wangjun, Yang Wenhui, Shen Congang, Kang Yichen, Qin Shuhao
State Key Laboratory of Aridland Crop Science, College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel). 2025 Jun 23;14(13):1921. doi: 10.3390/plants14131921.
Saline-alkali stress severely inhibited potato growth, yield, and quality, and exogenous abscisic acid (ABA) played an important role in plant stress resistance. In this study, potato tissue culture seedlings were used as experimental materials, the control group was cultured in the MS medium without adding any substances, and the treatment group was cultured in MS medium supplemented with 50 mmol/L NaHCO or 50 mmol/L NaHCO + 38 µM ABA, respectively. To explore the effect of exogenous ABA on the biological characteristics of potato plants under saline-alkali stress, a genetic improvement strategy was designed based on PP2C (PGSC0003DMT400046381), a key gene of the ABA signaling pathway. The results showed that saline-alkali stress led to leaf greening, wilting, and root development stunting, while exogenous ABA treatment significantly alleviated stress damage. PP2C negatively regulates ABA signaling. SnRK2s are activated when PP2Cs are inactivated during the ABA response. Compared with wild-type CK, it was found that TG lines had increased SOD and POD activities, increased carotenoid and ABA contents, reduced the increase in Na content and the decrease in K content, and interfered with PP2C (PGSC0003DMT400046381) to significantly enhance potato salinity-alkali resistance. This study provides a theoretical basis and technical path for the analysis of ABA-mediated plant stress resistance mechanism and the breeding of potato stress resistance varieties.
盐碱胁迫严重抑制马铃薯的生长、产量和品质，而外源脱落酸（ABA）在植物抗逆性中发挥重要作用。本研究以马铃薯组培苗为试验材料，对照组在不添加任何物质的MS培养基中培养，处理组分别在添加50 mmol/L NaHCO₃或50 mmol/L NaHCO₃ + 38 µM ABA的MS培养基中培养。为探究外源ABA对盐碱胁迫下马铃薯植株生物学特性的影响，基于ABA信号通路的关键基因PP2C（PGSC0003DMT400046381）设计了遗传改良策略。结果表明，盐碱胁迫导致叶片变绿、萎蔫和根系发育受阻，而外源ABA处理显著减轻了胁迫损伤。PP2C负调控ABA信号。在ABA应答过程中，当PP2C失活时SnRK2s被激活。与野生型CK相比，发现TG系超氧化物歧化酶（SOD）和过氧化物酶（POD）活性增加，类胡萝卜素和ABA含量增加，Na含量的增加和K含量的降低受到抑制，并干扰PP2C（PGSC0003DMT400046381）以显著增强马铃薯的抗盐碱能力。本研究为分析ABA介导的植物抗逆机制及马铃薯抗逆品种选育提供了理论依据和技术途径。
