Gao Xuan, Qian Hongyu, Huang Rui, He Wangyi, Jiang Haodong, Shen Ao, Li Zhi, Hu Yufu
College of Resources, Sichuan Agricultural University, Chengdu 611130, China.
Plants (Basel). 2025 Jun 23;14(13):1925. doi: 10.3390/plants14131925.
The rhizosphere serves as a critical interface for plant-soil-microorganism interactions. Rhizosphere soil refers to the soil directly adhering to root surfaces, while non-rhizosphere soil denotes the surrounding soil not in direct contact with roots. This study investigated the characteristics of soil microbial community structure, diversity, and enzyme activity dynamics in both rhizosphere and non-rhizosphere soils of (shrub) across different restoration periods (4, 8, 16, and 24 years) in alpine sandy lands on the eastern Qinghai-Tibet Plateau, with unrestored sandy land as control (CK), while analyzing relationships between soil properties and microbial characteristics. Results demonstrated that with increasing restoration duration, activities of sucrase, urease, alkaline phosphatase, and catalase in rhizosphere showed increasing trends across periods, with rhizosphere enzyme activities consistently exceeding non-rhizosphere levels. Bacterial Chao1 and Shannon indices followed similar patterns to enzyme activities, revealing statistically significant differences between rhizosphere and non-rhizosphere soils after 8 and 24 years of restoration, respectively. Dominant bacterial phyla ranked by relative abundance were Actinobacteria > Proteobacteria > Acidobacteria > Chloroflexi > Gemmatimonadetes. The relative abundance of Actinobacteria exhibited highly significant positive correlations with carbon, nitrogen, phosphorus, and enzyme activity indicators, indicating that restoration promoted improvements in soil physicochemical properties and nutrient accumulation, thereby enhancing bacterial community diversity and increasing Actinobacteria abundance. These findings provide fundamental data for restoration ecology and microbial ecology in alpine ecosystems, offering a scientific basis for optimizing ecological restoration processes and improving recovery efficiency in alpine sandy ecosystems.
根际是植物 - 土壤 - 微生物相互作用的关键界面。根际土壤是指直接附着在根表面的土壤，而非根际土壤则是指不与根直接接触的周围土壤。本研究调查了青藏高原东部高寒沙地不同恢复时期（4年、8年、16年和24年）[灌木名称未给出]根际和非根际土壤中土壤微生物群落结构、多样性及酶活性动态特征，以未恢复的沙地作为对照（CK），同时分析了土壤性质与微生物特征之间的关系。结果表明，随着恢复时间的增加，[灌木名称未给出]根际土壤中蔗糖酶、脲酶、碱性磷酸酶和过氧化氢酶的活性在各时期均呈上升趋势，根际酶活性始终高于非根际水平。细菌Chao1指数和香农指数与酶活性呈现相似模式，分别在恢复8年和24年后，根际和非根际土壤之间存在统计学显著差异。按相对丰度排序的优势细菌门类为放线菌门>变形菌门>酸杆菌门>绿弯菌门>芽单胞菌门。放线菌门的相对丰度与碳、氮、磷及酶活性指标呈极显著正相关，表明[灌木名称未给出]恢复促进了土壤理化性质的改善和养分积累，从而提高了细菌群落多样性并增加了放线菌门的丰度。这些研究结果为高寒生态系统的恢复生态学和微生物生态学提供了基础数据，为优化高寒沙地生态系统的生态恢复过程和提高恢复效率提供了科学依据。
