Verdú David, Valls Alicia, Serna-García Marta, Herrera Guadalupe, Ezzeddin-Ayoub Mustafa, Mauricio Maria D, Viña José, Serna Eva
Department of Physiology, Faculty of Medicine and Dentistry, CIBERFES, INCLIVA Biomedical Research Institute, University of Valencia, 46010 Valencia, Spain.
MODULAhR Group, University of Valencia, 46010 Valencia, Spain.
Int J Mol Sci. 2025 Jun 20;26(13):5957. doi: 10.3390/ijms26135957.
Aging is associated with increased oxidative stress, which contributes to cellular dysfunction and age-related diseases. Pomegranate extract (PE), rich in antioxidant polyphenols, may help mitigate oxidative damage. This study evaluated whether PE supplementation modulates oxidative stress by reducing reactive oxygen species (ROS) levels in white blood cells of aging mice. Aged mice (18 months) were supplemented with PE for four months, and cytoplasmic and mitochondrial ROS levels were assessed in leukocytes under basal conditions and oxidative stress conditions induced by tert-butyl hydroperoxide (t-BHP) using flow cytometry. Our results indicate that aged mice exhibit increased basal ROS levels in both the mitochondrial and cytoplasmic compartments, which were mitigated by PE supplementation. Furthermore, PE reversed the increase in hydrogen peroxide levels induced by τ-BHP and protected neutrophils by reducing mitochondrial ROS levels. These findings suggest that PE supplementation modulates the oxidative stress response, potentially improving immune function in aging. Given the central role of oxidative stress in age-related decline, PE may represent a valuable nutritional strategy to promote healthy aging.
衰老与氧化应激增加有关，氧化应激会导致细胞功能障碍和与年龄相关的疾病。富含抗氧化多酚的石榴提取物（PE）可能有助于减轻氧化损伤。本研究评估了补充PE是否通过降低衰老小鼠白细胞中的活性氧（ROS）水平来调节氧化应激。对18个月大的老年小鼠补充PE四个月，并使用流式细胞术在基础条件和叔丁基过氧化氢（t-BHP）诱导的氧化应激条件下评估白细胞中的细胞质和线粒体ROS水平。我们的结果表明，老年小鼠的线粒体和细胞质区室中的基础ROS水平均升高，而补充PE可减轻这种升高。此外，PE逆转了τ-BHP诱导的过氧化氢水平升高，并通过降低线粒体ROS水平保护中性粒细胞。这些发现表明，补充PE可调节氧化应激反应，可能改善衰老过程中的免疫功能。鉴于氧化应激在与年龄相关的衰退中起核心作用，PE可能是促进健康衰老的一种有价值的营养策略。
