Marijuán-Pinel Daniel, Mercader-Ruiz Jon, Beitia Maider, Sánchez Pello, López de Dicastillo Leonor, Gonzalez Sergio, Espregueira-Mendes João, Aizpurua Beatriz, Oraá Jaime, Delgado Diego, Sánchez Mikel
Advanced Biological Therapy Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain.
Arthroscopic Surgery Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain.
Int J Mol Sci. 2025 Jun 21;26(13):5967. doi: 10.3390/ijms26135967.
This study investigated the impact of increased extraplatelet content on the tissue regenerative capacity of platelet-rich plasma (PRP)-derived fibrin scaffolds. Comparative analyses were performed between a "balanced protein-concentrate plasma" (BPCP) and a standard PRP (sPRP), focusing on platelet and fibrinogen content, scaffold microstructure, and functional performance. Growth factor (GF) release kinetics from the scaffolds were quantified via ELISA over 10 days, while scaffold biomechanics were evaluated through rheological testing, indentation, energy dissipation, adhesion, and assessments of coagulation dynamics, biodegradation, swelling, and retraction. Microstructural analysis was conducted using scanning electron microscopy (SEM), with fiber diameter and porosity measurements. The results demonstrated that BPCP scaffolds released significantly higher amounts of GFs and total protein, especially beyond 24 h (* < 0.05). Despite a delayed coagulation process (** < 0.01), BPCP scaffolds exhibited superior structural integrity and cushioning behavior (* < 0.05). SEM revealed thicker fibers in BPCP scaffolds (**** < 0.0001), while adhesion and biodegradation remained unaffected. Notably, BPCP scaffolds showed reduced retraction after 24 h and maintained their shape stability over two weeks without significant swelling. These findings indicate that enhancing the extraplatelet content in PRP formulations can optimize fibrin scaffold performance. Further preclinical and clinical studies are warranted to evaluate the therapeutic efficacy of BPCP-derived scaffolds in regenerative medicine.
本研究调查了增加血小板外成分含量对富血小板血浆（PRP）衍生纤维蛋白支架组织再生能力的影响。对“平衡蛋白浓缩血浆”（BPCP）和标准PRP（sPRP）进行了比较分析，重点关注血小板和纤维蛋白原含量、支架微观结构及功能性能。通过酶联免疫吸附测定（ELISA）对支架生长因子（GF）释放动力学进行了为期10天的定量分析，同时通过流变学测试、压痕测试、能量耗散测试、黏附测试以及凝血动力学、生物降解、肿胀和回缩评估对支架生物力学进行了评价。使用扫描电子显微镜（SEM）进行微观结构分析，并测量纤维直径和孔隙率。结果表明，BPCP支架释放的GF和总蛋白量显著更高，尤其是在24小时后（*P<0.05）。尽管凝血过程延迟（**P<0.01），但BPCP支架表现出卓越的结构完整性和缓冲性能（*P<0.05）。SEM显示BPCP支架中的纤维更粗（****P<0.0001），而黏附性和生物降解性未受影响。值得注意的是，BPCP支架在24小时后回缩减少，并在两周内保持形状稳定性，无明显肿胀。这些发现表明，提高PRP制剂中的血小板外成分含量可优化纤维蛋白支架性能。有必要开展进一步的临床前和临床研究，以评估BPCP衍生支架在再生医学中的治疗效果。
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