• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

CAT2精氨酸转运蛋白的克隆、表达及功能特性分析

Cloning, Expression and Functional Characterization of CAT2 Arginine Transporter.

作者信息

Pochini Lorena, Regina Teresa Maria Rosaria, Cerbelli Maria Iolanda, Gallo Nicoletta, Costantino Federica, Galluccio Michele, Indiveri Cesare

机构信息

Laboratory of Biochemistry, Molecular Biotechnology and Molecular Biology, Department of Biology, Ecology and Earth Sciences (DiBEST), University of Calabria, Via P. Bucci 4c, 6c, 87036 Arcavacata di Rende, Cosenza, Italy.

Institute of Biomembranes, Bioenergetics and Molecular Biotechnology (IBIOM), National Research Council of Italy (CNR), Via G. Amendola 122/O, 70126 Bari, Italy.

出版信息

Int J Mol Sci. 2025 Jun 28;26(13):6259. doi: 10.3390/ijms26136259.

DOI:10.3390/ijms26136259
PMID:40650036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12250071/
Abstract

The amino acid membrane transporters of grape species take part in metabolic pathways that play crucial roles in nitrogen trafficking and in the synthesis of secondary metabolites. Therefore, identifying these amino acid transporters and defining their functional properties might have further applications in crop improvement and, hence, relevance to human nutrition. The (Cation Amino acid Transporter) transporter cDNA has been isolated and cloned into a specific plasmid for over-expression in . The expressed protein, after purification by Ni-chelating chromatography, has been functionally characterized in an experimental model of proteoliposomes by measuring the uptake of radiolabeled compounds. Arginine was revealed to be the best substrate, confirming the role of CAT2 in nitrogen trafficking in plant cells and within sub-cellular spaces, given its plausible localization in vacuoles. The transporter activity is modulated by pH, osmotic imbalance and ATP. The transport kinetics have been measured. Overall, the obtained data indicate the capacity of CAT2 in transporting arginine, making it a possible target for crop improvement with a relevance to human health.

摘要

葡萄品种的氨基酸膜转运蛋白参与了在氮素转运和次生代谢物合成中起关键作用的代谢途径。因此，鉴定这些氨基酸转运蛋白并确定其功能特性可能在作物改良中具有进一步的应用，从而与人类营养相关。已分离出（阳离子氨基酸转运蛋白）转运体cDNA，并将其克隆到特定质粒中以便在[具体生物]中过表达。通过镍螯合层析纯化后的表达蛋白，已在蛋白脂质体实验模型中通过测量放射性标记化合物的摄取进行了功能表征。精氨酸被证明是最佳底物，鉴于其可能定位于液泡，这证实了CAT2在植物细胞和亚细胞空间中氮素转运中的作用。转运体活性受pH、渗透失衡和ATP调节。已测量了转运动力学。总体而言，所获得的数据表明CAT2具有转运精氨酸的能力，使其成为与人类健康相关的作物改良的可能靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/eb2ccfe55efa/ijms-26-06259-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/5ed29ae6a58b/ijms-26-06259-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/a63fb128af64/ijms-26-06259-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/dc736294411a/ijms-26-06259-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/348b9f7aabe1/ijms-26-06259-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/702d05aee28d/ijms-26-06259-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/be881919cd85/ijms-26-06259-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/0734179f1d4d/ijms-26-06259-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/50b33b327a7b/ijms-26-06259-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/440b69edf173/ijms-26-06259-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/5d66a8f32ff4/ijms-26-06259-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/eb2ccfe55efa/ijms-26-06259-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/5ed29ae6a58b/ijms-26-06259-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/a63fb128af64/ijms-26-06259-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/dc736294411a/ijms-26-06259-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/348b9f7aabe1/ijms-26-06259-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/702d05aee28d/ijms-26-06259-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/be881919cd85/ijms-26-06259-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/0734179f1d4d/ijms-26-06259-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/50b33b327a7b/ijms-26-06259-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/440b69edf173/ijms-26-06259-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/5d66a8f32ff4/ijms-26-06259-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e3bf/12250071/eb2ccfe55efa/ijms-26-06259-g011.jpg

相似文献

1
Cloning, Expression and Functional Characterization of CAT2 Arginine Transporter.CAT2精氨酸转运蛋白的克隆、表达及功能特性分析
Int J Mol Sci. 2025 Jun 28;26(13):6259. doi: 10.3390/ijms26136259.
2
[Volume and health outcomes: evidence from systematic reviews and from evaluation of Italian hospital data].[容量与健康结果：来自系统评价和意大利医院数据评估的证据]
Epidemiol Prev. 2013 Mar-Jun;37(2-3 Suppl 2):1-100.
3
Management of urinary stones by experts in stone disease (ESD 2025).结石病专家对尿路结石的管理（2025年结石病专家共识）
Arch Ital Urol Androl. 2025 Jun 30;97(2):14085. doi: 10.4081/aiua.2025.14085.
4
The Black Book of Psychotropic Dosing and Monitoring.《精神药物剂量与监测黑皮书》
Psychopharmacol Bull. 2024 Jul 8;54(3):8-59.
5
The clinical effectiveness and cost-effectiveness of enzyme replacement therapy for Gaucher's disease: a systematic review.戈谢病酶替代疗法的临床疗效和成本效益：一项系统评价。
Health Technol Assess. 2006 Jul;10(24):iii-iv, ix-136. doi: 10.3310/hta10240.
6
Short-Term Memory Impairment短期记忆障碍
7
A protocol for cloning, expression, and purification of Lysine exporter (LysE) gene of Mycobacterium tuberculosis.结核分枝杆菌赖氨酸外排蛋白（LysE）基因的克隆、表达及纯化方案。
F1000Res. 2024 Feb 5;12:297. doi: 10.12688/f1000research.131768.2. eCollection 2023.
8
Isolated Methylmalonic Acidemia孤立性甲基丙二酸血症
9
A rapid and systematic review of the clinical effectiveness and cost-effectiveness of paclitaxel, docetaxel, gemcitabine and vinorelbine in non-small-cell lung cancer.对紫杉醇、多西他赛、吉西他滨和长春瑞滨在非小细胞肺癌中的临床疗效和成本效益进行的快速系统评价。
Health Technol Assess. 2001;5(32):1-195. doi: 10.3310/hta5320.
10
What is the value of routinely testing full blood count, electrolytes and urea, and pulmonary function tests before elective surgery in patients with no apparent clinical indication and in subgroups of patients with common comorbidities: a systematic review of the clinical and cost-effective literature.在没有明显临床指征的患者和常见合并症患者亚组中，在择期手术前常规检测全血细胞计数、电解质和尿素以及肺功能测试的价值：对临床和成本效益文献的系统评价。
Health Technol Assess. 2012 Dec;16(50):i-xvi, 1-159. doi: 10.3310/hta16500.

本文引用的文献

1
Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes ( L.) Under Abiotic Stress and During Development.非生物胁迫及发育过程中葡萄（Vitis vinifera L.）氨基酸/生长素转运蛋白（AAAP）基因的全基因组鉴定与表达分析
Plants (Basel). 2025 Jan 4;14(1):128. doi: 10.3390/plants14010128.
2
Genome-Wide Identification of the Genes and Molecular Characterization of Their Transcriptional Responses to Various Nutrient Stresses in Allotetraploid Rapeseed.异源四倍体油菜中响应各种营养胁迫的基因全基因组鉴定及其转录反应的分子特征分析
Int J Mol Sci. 2024 Nov 25;25(23):12658. doi: 10.3390/ijms252312658.
3
Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.
植物中膜转运蛋白的类型及其与活性氧之间的相互作用机制
Antioxidants (Basel). 2024 Feb 9;13(2):221. doi: 10.3390/antiox13020221.
4
Bacterial over-production of the functionally active human SLC38A2 (SNAT2) exploiting the mistic tag: a cheap and fast tool for testing ligands.利用 mistic 标签实现功能性人源 SLC38A2（SNAT2）的细菌过表达：一种廉价且快速的测试配体的工具。
Mol Biol Rep. 2024 Feb 23;51(1):336. doi: 10.1007/s11033-023-08976-3.
5
Resveratrol for the Management of Human Health: How Far Have We Come? A Systematic Review of Resveratrol Clinical Trials to Highlight Gaps and Opportunities.白藜芦醇在人类健康管理中的应用：我们已经走了多远？系统综述白藜芦醇临床试验，以突显差距和机遇。
Int J Mol Sci. 2024 Jan 6;25(2):747. doi: 10.3390/ijms25020747.
6
Over-Production of the Human SLC7A10 in and Functional Assay in Proteoliposomes.在质体蓝素体中过度表达人类 SLC7A10 及其功能分析。
Int J Mol Sci. 2023 Dec 30;25(1):536. doi: 10.3390/ijms25010536.
7
Comprehensive genomic characterization of cotton cationic amino acid transporter genes reveals that GhCAT10D regulates salt tolerance.全面的棉花阳离子氨基酸转运蛋白基因的基因组特征分析表明 GhCAT10D 调控耐盐性。
BMC Plant Biol. 2022 Sep 15;22(1):441. doi: 10.1186/s12870-022-03829-w.
8
Bacterial over-expression of functionally active human CT2 (SLC22A16) carnitine transporter.细菌中功能活跃的人 CT2（SLC22A16）肉碱转运蛋白的过度表达。
Mol Biol Rep. 2022 Aug;49(8):8185-8193. doi: 10.1007/s11033-022-07491-1. Epub 2022 May 24.
9
Tonoplast-Localized Theanine Transporter CsCAT2 May Mediate Theanine Storage in the Root of Tea Plants ( L.).液泡膜定位的茶氨酸转运蛋白CsCAT2可能介导茶树根中茶氨酸的储存。
Front Plant Sci. 2021 Dec 17;12:797854. doi: 10.3389/fpls.2021.797854. eCollection 2021.
10
The Sterol Trafficking Pathway in .……中的甾醇转运途径
Front Plant Sci. 2021 May 26;12:616631. doi: 10.3389/fpls.2021.616631. eCollection 2021.