Du Xiao-Qian, Sun Si-Si, Zhou Ting, Zhang Lu, Feng Ying-Na, Zhang Kun-Long, Hua Ying-Peng
School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Int J Mol Sci. 2024 Nov 25;25(23):12658. doi: 10.3390/ijms252312658.
is an important oil crop in China and has a great demand for nitrogen nutrients. Cationic amino acid transporters (CAT) play a key role in amino acid absorption and transport in plants. However, the family has not been reported in so far. In this study, genome-wide analysis identified 22 members in the genome. Based on phylogenetic and synteny analysis, were classified into four groups (Group I-Group IV). The members in the same subgroups showed similar physiochemical characteristics and intron/exon and motif patterns. By evaluating -elements in the promoter regions, we identified some -elements related to hormones, stress and plant development. Darwin's evolutionary analysis indicated that might have experienced strong purifying selection pressure. The family may have undergone gene expansion; the chromosomal location of indicated that whole-genome replication or segmental replication may play a major driving role. Differential expression patterns of under nitrate limitation, phosphate shortage, potassium shortage, cadmium toxicity, ammonium excess and salt stress conditions indicated that they were responsive to different nutrient stresses. In summary, these findings provide a comprehensive survey of the family and lay a foundation for the further functional analysis of family members.
是中国重要的油料作物,对氮素营养需求巨大。阳离子氨基酸转运体(CAT)在植物氨基酸吸收和转运中起关键作用。然而,该家族在[具体物种]中迄今尚未见报道。本研究通过全基因组分析在[具体物种]基因组中鉴定出22个CAT成员。基于系统发育和共线性分析,CAT被分为四组(第一组 - 第四组)。同一亚组的成员表现出相似的理化特性以及内含子/外显子和基序模式。通过评估启动子区域中的顺式作用元件,我们鉴定出一些与激素、胁迫和植物发育相关的顺式作用元件。达尔文进化分析表明CAT可能经历了强烈的纯化选择压力。CAT家族可能经历了基因扩张;CAT的染色体定位表明全基因组复制或片段复制可能起主要驱动作用。CAT在硝酸盐限制、磷缺乏、钾缺乏、镉毒性、铵过量和盐胁迫条件下的差异表达模式表明它们对不同的营养胁迫有响应。总之,这些发现对CAT家族进行了全面的调查,并为进一步对家族成员进行功能分析奠定了基础。