Genome-Wide Identification and Expression Analysis of Amino Acid/Auxin Permease (AAAP) Genes in Grapes ( L.) Under Abiotic Stress and During Development.

Amino acids in wine grapes function as precursors for various secondary metabolites and play a vital role in plant growth, development, and stress resistance. The amino acid/auxin permease () genes encode a large family of transporters; however, the identification and function of the gene family in grapes remain limited. Consequently, we conducted a comprehensive bioinformatics analysis of all genes in grapes, encompassing genome sequence analysis, conserved protein domain identification, chromosomal localization, phylogenetic relationship analysis, and gene expression profiling. This study identified 60 genes, distributed on 14 chromosomes and classified into eight subfamilies. Microarray and transcriptome data revealed that most genes decrease during development, but and gradually increase. and exhibited significantly higher expression levels, while demonstrated lower expression when subjected to salt and drought stress. genes exhibited diverse expression patterns, suggesting that the gene family possesses both diversity and specific functions in grapes. Furthermore, the expression patterns of genes analyzed by RT-qPCR facilitate further investigation into the biological functions of individual genes in different tissues. These findings provide valuable insights into the continued analysis of the gene family's functions in grapes.