Yu Yanan, Chang Qihang, Li Chunyue, Wu Kaiyue, Wang Yanyan, Guo Changhong, Shu Yongjun, Bai Yan
Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.
Department of Biomedical Engineering, School of Medical Devices, Shenyang Pharmaceutical University, Shenyang 110016, China.
Int J Mol Sci. 2025 Jun 29;26(13):6289. doi: 10.3390/ijms26136289.
The SHORT INTERNODES-related sequence (SRS) gene family, comprising zinc finger and IXGH domain-containing transcription factors, serves as a critical regulator of plant biological processes and abiotic stress responses. In this study, the common wheat cultivar Chinese Spring was selected as the experimental material. Comprehensive bioinformatic analysis was performed using ClustalX, MEGA, MEME, and PlantTFDB v5.0 to systematically characterize SRS family members within the wheat genome. The systematic examination of physicochemical properties, conserved domains, phylogenetic relationships, gene structures, and cis-acting elements was conducted, providing insights into the functional roles of this gene family in wheat growth and development. Fifteen SRS family members containing conserved zinc finger and IXGH domains were identified. Distinct expression patterns were observed among subgroups: Members of Groups I, III, and V exhibited significantly higher transcript levels in roots, stems, leaves, and anthers compared to other subgroups. Notably, the majority of genes, including representatives from Groups I, III, IV, and V, displayed responsiveness to NaCl and ABA stress treatments, suggesting their putative involvement in both salinity adaptation and phytohormone-mediated stress signaling. Differential expression patterns of genes under NaCl and ABA stress were identified, revealing distinct regulatory impacts of these stressors on transcription. These findings establish a framework for investigating the molecular mechanisms underlying stress adaptation in wheat physiology.
与短节间相关的序列（SRS）基因家族，由包含锌指和IXGH结构域的转录因子组成，是植物生物学过程和非生物胁迫响应的关键调节因子。在本研究中，选择普通小麦品种中国春作为实验材料。使用ClustalX、MEGA、MEME和PlantTFDB v5.0进行了全面的生物信息学分析，以系统地表征小麦基因组中的SRS家族成员。对理化性质、保守结构域、系统发育关系、基因结构和顺式作用元件进行了系统研究，从而深入了解该基因家族在小麦生长发育中的功能作用。鉴定出15个含有保守锌指和IXGH结构域的SRS家族成员。在各亚组中观察到不同的表达模式：与其他亚组相比，第I、III和V组的成员在根、茎、叶和花药中的转录水平显著更高。值得注意的是，大多数基因，包括来自第I、III、IV和V组的代表基因，对NaCl和ABA胁迫处理有响应，表明它们可能参与盐适应性和植物激素介导的胁迫信号传导。鉴定了NaCl和ABA胁迫下基因的差异表达模式，揭示了这些胁迫因子对转录的不同调节影响。这些发现为研究小麦生理学中胁迫适应的分子机制建立了一个框架。
