Torices-Hernández Álvaro, Gallego Marta, Mora Leticia, Toldrá Fidel
Instituto de Agroquimica y Tecnologia de Alimentos (IATA-CSIC), Avenue Agustin Escardino 7, 46980 Paterna, Spain.
Int J Mol Sci. 2025 Jun 30;26(13):6344. doi: 10.3390/ijms26136344.
Chicken feathers constitute a major by-product from the poultry industry, with a potential environmental impact and significant difficulties in their management. This study aimed to develop a sustainable method to hydrolyse chicken feathers and evaluate the effects of microwave (MW) irradiation pre-treatment in the generation of bioactive hydrolysates by simple or sequential hydrolysis with Alcalase. The hydrolysate with MW irradiation pre-treatment and Alcalase (2%, 2 h) (MWA) showed the highest overall antioxidant activity and neprilysin-inhibitory activity (55%), whereas samples without MW irradiation pre-treatment exerted the highest inhibitory activity of dipeptidyl peptidase IV (DPP IV) and angiotensin-converting enzyme (ACE-I), with values close to 50 and 70%, respectively. Mass spectrometry in tandem of bioactive hydrolysates was performed, and an in silico approach was used to characterise the obtained sequences. These results confirmed that MW irradiation pre-treatment improved Alcalase hydrolysis, leading to the generation of bioactive peptides with potential multifunctional properties, including antioxidant, antidiabetic, and antihypertensive activities. Moreover, this study highlights the potential of combining MW irradiation and enzymatic hydrolysis as a sustainable strategy for the revalorisation of chicken feathers.
鸡毛是家禽业的一种主要副产品，对环境有潜在影响且管理难度较大。本研究旨在开发一种可持续的鸡毛水解方法，并评估微波（MW）辐照预处理对通过用碱性蛋白酶进行简单或顺序水解生成生物活性水解产物的影响。经MW辐照预处理并用碱性蛋白酶（2%，2小时）处理的水解产物（MWA）显示出最高的总抗氧化活性和中性内肽酶抑制活性（55%），而未经MW辐照预处理的样品对二肽基肽酶IV（DPP IV）和血管紧张素转换酶（ACE-I）的抑制活性最高，分别接近50%和70%。对生物活性水解产物进行了串联质谱分析，并采用计算机模拟方法对获得的序列进行了表征。这些结果证实，MW辐照预处理改善了碱性蛋白酶的水解效果，从而产生了具有潜在多功能特性的生物活性肽，包括抗氧化、抗糖尿病和抗高血压活性。此外，本研究突出了将MW辐照与酶水解相结合作为鸡毛增值可持续策略的潜力。
