Bondi Edoardo, Restivo Elisa, Soccio Michelina, Guidotti Giulia, Bloise Nora, Motta Ilenia, Gazzano Massimo, Ruggeri Marco, Fassina Lorenzo, Visai Livia, Pasquinelli Gianandrea, Lotti Nadia
Department of Civil, Chemical, Environmental, and Materials Engineering, University of Bologna, Via Terracini 28, 40131 Bologna, Italy.
Molecular Medicine Department (DMM), Centre for Health Technologies (CHT), Unità di Ricerca (UdR) INSTM, Operative Unit (OU) of Interuniversity Center for the Promotion of the 3Rs Principles in Teaching and Research (Centro 3R), University of Pavia, 27100 Pavia, Italy.
Int J Mol Sci. 2025 Jul 4;26(13):6470. doi: 10.3390/ijms26136470.
Cardiovascular diseases are responsible for a large number of severe disability cases and deaths worldwide. Strong research in this field has been extensively carried out, in particular for the associated complications, such as the occlusion of small-diameter (<6 mm) vessels. Accordingly, in the present research, two random copolyesters of poly(butylene 2,5-furandicarboxylate) (PBF) and poly(butylene isophthalate) (PBI), were successfully synthesized via two-step melt polycondensation and were thoroughly characterized from molecular, thermal, and mechanical perspectives. The copolymeric films displayed a peculiar thermal behavior, being easily processable in the form of films, although amorphous, with T close to room temperature. Their thermal stability was high in all cases, and from the mechanical point of view, the materials exhibited a high ultimate strength, together with values of elastic moduli tunable with the chemical composition. The long-term stability of these materials under physiological conditions was also demonstrated. Cytotoxicity was assessed using a direct contact assay with human umbilical vein endothelial cells (HUVECs). In addition, hemocompatibility was tested by evaluating the adhesion of blood components (such as the adsorption of human platelets and fibrinogen). As a result, a proper chemical design and, in turn, both the solid-state and functional properties, are pivotal in regulating cell behavior and opening new frontiers in the tissue engineering of soft tissues, including vascular tissues.
心血管疾病在全球范围内导致了大量严重残疾病例和死亡。该领域已开展了大量深入研究，特别是针对相关并发症，如小直径（<6 mm）血管闭塞。因此，在本研究中，通过两步熔融缩聚成功合成了两种聚（2,5-呋喃二甲酸丁二醇酯）（PBF）和聚（间苯二甲酸丁二醇酯）（PBI）的无规共聚酯，并从分子、热学和力学角度对其进行了全面表征。共聚物薄膜呈现出独特的热行为，尽管为无定形，但易于制成薄膜形式，其玻璃化转变温度接近室温。在所有情况下，它们的热稳定性都很高，从力学角度来看，这些材料具有较高的极限强度，同时弹性模量值可随化学组成调节。还证明了这些材料在生理条件下的长期稳定性。使用与人类脐静脉内皮细胞（HUVECs）的直接接触试验评估细胞毒性。此外，通过评估血液成分的粘附（如人类血小板和纤维蛋白原的吸附）来测试血液相容性。结果表明，合理的化学设计以及固态和功能特性，对于调节细胞行为和开拓软组织（包括血管组织）组织工程的新领域至关重要。
