Zhang Yuejing, Wang Xin, Sang Hetian, Cai Yuting, Pang Hailong, Jia Lingyun, Li Wenliang, Feng Hanqing
College of Life Science, Northwest Normal University, Lanzhou, Gansu, China.
Key Laboratory of Biological Resources Development and Biological Products in the Northwest Plateau, China General Chamber of Commerce, Lanzhou, Gansu, China.
Physiol Plant. 2025 Jul-Aug;177(4):e70381. doi: 10.1111/ppl.70381.
In Arabidopsis (Arabidopsis thaliana), the lectin receptor kinases I.9 (P2K1) and I.5 (P2K2) are known as the receptors of extracellular ATP (eATP) and play important roles in the regulation of the responses of plants to environmental stresses. In the presented work, the possible involvement of these receptors of eATP in regulating local and systemic ROS (reactive oxygen species) accumulation to local salt stress was investigated. The roots or a single leaf of the seedlings were subjected to local salt stress, and the leaves that did not directly experience salt stress were regarded as the systemic organs. The results showed that local treatment with salt stress caused a local and systemic increase in eATP levels. Local application of exogenous ATP to the roots or a single leaf can evoke local and systemic ROS accumulation. Interestingly, local salt stress-induced local accumulation of ROS was weakened by P2K mutations, which also attenuated the systemic accumulation of ROS induced by local salt stress. In addition, local and systemic increases in HO (hydrogen peroxide) content and CAT (catalase) activity by local NaCl stress were also reduced by the P2K mutations. These results suggest that the eATP receptors play an important role in regulating local and systemic ROS accumulation in response to local salt stress.
在拟南芥（Arabidopsis thaliana）中，凝集素受体激酶I.9（P2K1）和I.5（P2K2）作为细胞外ATP（eATP）的受体，在调节植物对环境胁迫的反应中发挥重要作用。在本研究中，研究了这些eATP受体在调节局部和系统性活性氧（ROS）积累以应对局部盐胁迫中的可能作用。对幼苗的根或一片叶子施加局部盐胁迫，未直接经历盐胁迫的叶子被视为系统性器官。结果表明，盐胁迫局部处理导致eATP水平局部和系统性升高。向根或一片叶子局部施加外源ATP可引发局部和系统性ROS积累。有趣的是，P2K突变削弱了局部盐胁迫诱导的局部ROS积累，这也减弱了局部盐胁迫诱导的系统性ROS积累。此外，P2K突变还降低了局部NaCl胁迫导致的局部和系统性过氧化氢（HO）含量增加以及过氧化氢酶（CAT）活性增加。这些结果表明，eATP受体在调节局部盐胁迫响应中局部和系统性ROS积累方面发挥重要作用。
